Acid Phosphatase 8

Total Acid Phosphatase

• Specimen: 0.1 ml (100 (II)
• Total ACP working solution : 1.0 ml

Non Prostatic Acid Phosphatase

• Specimen: 0.1 ml (100 gl)
• Non Prostatic ACP working solution :1.0 ml

For each assay, mix thoroughly and transfer the assay mixture immediately to the thermo stated cuvette and start the stop watch simultaneously. Record the first reading at 300th second and subsequently three more readings with 60 seconds interval at 405 nm.

EXPECTED VALUES

• Total Acid Phosphatase : up to 4.7 IU/1
• Prostatic Acid Phosphatase: up to 1.6 IU/1

NOTE :

Since the expected values are affected by age, sex, diet and geographical location, each laboratory is strongly urged to establish its own reference range for this procedure.

PROCEDURE LIMITATIONS

Working reagent is considered unsatisfactory and should not be used if its absorbance exceeds 0.500 at 405 nm. against distilled water.

If the Acid Phosphatase activity is above 75 IU/1 then dilute the specimen suitably with normal saline. In such case the results obtained should be multiplied by dilution factor to obtain correct Acid Phosphatase activity.

QUALITY CONTROL

To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realised that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.