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ADA Calibrator

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ADA Calibrator
ADA Calibrator
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 5943 INR
    Product Specifications
    • other
    • Biochemistry Reagent
    • Other
    • Biochemistry Reagent
    • Other
    • 18 Months
    • Infinite ADA is a reagent set for direct quantitative determination of Adenosine deaminase (ADA) activity in human serum, plasma and other body fluids. Infinite ADA is a ready-to-use, two liquid reagent system.
    • Liquid
    • Reagents
    • Other
    • ACC90498
    • Industrial
    • Industrial Lab Chemicals
    • 95%
      Product Description
      INTRODUCTION 
      •  Infinite ADA is a reagent set for direct quantitative  determination of Adenosine deaminase (ADA) activity in  human serum, plasma and other body fluids. 
      •  Infinite ADA is a ready-to-use, two liquid reagent system. 
      • With Infinite ADA, the assay is linear upto 200 U/L. 
      • Infinite ADA assay can be performed in 11 minutes at  37 C. 
      PRINCIPLE

      ADA catalyses deamination of adenosine to inosine which is then converted to hypoxanthine by purine nucleosidephosphorylase (PNP). Hypoxanthine is then converted to uric acid and hydrogen peroxide (H2O2) by xanthine oxidase  (XOD). H2O2 is further reacted with N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline (EHSPT) and 4-aminoantipyrine  (4 AAP) in the presence of peroxidase (POD) to generate  quinone dye.

      denosine + H2O Inosine + NH3 PNP Inosine + Pi Hypoxanthine + Ribose-1-phosphate XOD Hypoxanthine + 2H2O + 2O2 Uric acid + 2 H2O2 POD 2 H2O2 + 4AAP + EHSPT 4 H2O + Quinone dye  

      The intensity of the colour developed is directly proportional to the activity of ADA in the specimen and is measured kinetically.  

      REAGENT STORAGE,  STABILITY & HANDLING 

      • The reagents R1 & R2 are ready-to-use. 
      • The reagent kit should be stored at 2 Degree- 8 Degree C and is stable till 
      • the expiry date indicated on the label. 
      • Reagent R1 is light-sensitive & to be stored in dark. 
      • Contamination of the reagents should be strictly avoided. 
      COMPONENTS & CONCENTRATION OF WORKING SOLUTION 

      Component Concentration 
       R1 
      • Tris 45mM 
      • PNP 0.08 U/ml 
      • Xanthine oxidase 0.15 U/ml 
      • Peroxidase 0.40 U/ml 
      • Stabilizers, excipients & surface active agents 
       R2 
      • Tris 40mM 
      • Adenosine 8 M 
      • EHSPT 1M

      SPECIMEN COLLECTION  & PRESERVATION 

      Blood should be collected in a clean dry container. Serum or  heparinized plasma may be used. Do not use citrate or oxalate as anticoagulant. Ideally, venous blood should be  collected & handled anaerobically. 

      It is recommended to use fresh sample. Serum or plasma,  after prompt separation from cells or clot, should be kept  tightly stoppered. Do not use hemolyzed, contaminated or  turbid samples.  Ideal collection procedure, i.e. disinfecting the site &  collecting with aseptic precautions, should be followed when  other body fluids (Pleural fluid, pericardial fluid, ascitic fluid, 
      cerebrospinal fluid) are tested. 

      ADA activity in serum/plasma is stable for 3 days when stored  at 2 Degree - 8 Degree C & in other body fluids for 2 days when stored at 2 Degree - 8 Degree C .  

      PROCEDURE

      • Reaction type :  Kinetic 
      • Reaction direction:  .Increasing 
      • Wavelength :  546 nm 
      • Flowcell temperature:  .37 Degree C 
      • Zero setting with:  Distilled water 
      • Delay time:  300 seconds 
      • No. of readings:   4 
      • Interval :   60 seconds 
      • Sample volume:  10 Ul 
      • R 1 volume:   360 Ul 
      • R 2 volume:  180 Ul 
      • Factor:   1743 
      • Linearity:   200 U/L 

      EXPECTED VALUES

      The indicative reference values for Indian population are as follows:

       

      Normal

      Suspect

      Strong Suspect

      Serum/plasma

       22U/L

      22 - 40U/L

       40U/L

      Body fluids (Pleural/Pericardial/Ascitic)

       30U/L

      30 - 40U/L

      40U/L

      CSF

       9U/L

      9 - 12 U/L

      12U/L

       

      Trade Information
      • EXW ABPL
      • Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
      • 10000 Per Day
      • 2 Days
      • Yes
      • packaging : Empity
      • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
      • All India
      • certification : iso
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      Contact Us

      212, Udyog Mandir No. 17/C, Bhagoji Keer Marg, Mahim, Mumbai, Maharashtra, 400016, India
      Phone :+918045479256