Alkaline Phosphatase 100

Alkaline Phosphatase 100
Alkaline Phosphatase 100
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 2702 INR
    Product Specifications
    • Industrial
    • Industrial Lab Chemicals
    • 95
    Product Description


    • AutoPure Alkaline Phosphatase is a reagent kit for direct quantitative determination of Alkaline Phosphatase  in human serum and plasma on automated clinical chemistry analyzers.
    •  AutoPure Alkaline Phosphatase is a singal reagent system.
    •  AutoPure Alkaline Phosphatase  has a singal step reconstitution . It involves reconstitution of substrate tablet with the diluent.
    • With  AutoPure Alkaline Phosphatase , the assay is linear upto 2000IU/I(33.4ukat/I)


     Alkaline Phosphatase cleaves p-nitrophenyl phosphate (p-NPP) in the presence of magnesium ions, to form p-nitrophenol and phosphate.

    p-nitrophenyl phosphate+ h20 p-nitrophenol+phosphate

    The intensity of the yellow colour formed is directly proportional to the  Alkaline Phosphatase activity in the specimen and is measured kinetically.

    Preparation Of Working Solution

     Reconstitute the substrate tablet with diluent as per the instruction indicated on the diluent bottle. Swirl gently.

    Reagent Storage, Stability & Handling 

     AutoPure Alkaline Phosphatase is a singal reagent system.

    Shelf life 

    Stable till the expiry date indicated on the label, when stored at 2 8 C

    On- Board Reagent Stability 

    21 days 2- 8 C after opening Protect the reagent from light and contamination.

    Do not freeze the reagent

    Components & Concentration Of  Working Solution

    Component                                                                               Concentration 

    • Diethanolamine buffer, ph9.8                                           1mol/I
    • p-nitrophenyl phosphate                                                  >10mmol/1
    • Magnesium Chloride                                                         >0.5 mmol/1
    • Stabilizers, excipients & surface active agents.                 

    Specimen Collection & Preservation

    Collect sample using standard sampling tube. Haemolyzed specimen should be avoided as it may falsely elevate results. Serum or heparinized plasma can be used. Anticogulants suc as EDTA oxalate or citrate inhibit the Alkaline Phosphatase activity and hence cannot be used. Alkaline Phosphatase  activity in serum/ plasma is stable for 4 days at 2- 8 C and 28 days at -20 C . Centrifuge samples containing precipitate before performing assay.


    Fully automated system automatically calculate the Alkaline Phosphatase activity of each sample.

    Result in ukat/l= Result in IU/Ix 0.0167

    Application Sheet 

    Refer to application sheet for details. For additional system application, contact our local Accurex representative.


    For calibration , it is recommended to use C.f,a,s or any other suitable calibrator material.

    Calibration frequency 

    Re- calibration is recommended

    • Whenever a fresh reconstituted reagent is used.
    • Whenever the reagent lot is changed.
    • As per the requirement of quality control procedures.

    Absorption of atmospheric CO2 by the opened reagent bottle leads to impaired calibration stability. 

    Quality Control

    Each batch of AutoPure   Alkaline Phosphatase is assayed with alteast six quality control sera Precinorm, Precipath, Biorad l, Accutrol Normal and Accutrol Abnormal prior to release.

    To ensure adequate control, it is recommended that the labortory should use a normal and abnormal commercial reference control the reagent and instrument functions together.

    If the control values fall outside the specified limits, each of the below criteria should be crosee checked and corrected:

    • Proper instrument function wavelength setting, light source and temperature control.
    • Cleanliness of probes and cuvettes.
    • Bacterial contamination of wash water used by the instrument.
    • Expiry date of the reagent kill

    Expected Values


    • Serum/Plasma
    • 37 C
    • Adult (More than 15 years)
    • 108-306 IU/I
    • 1.8- 5.1 ukat/I
    • Child (Less than 15 years)
    • 210-810IU/I
    • 3.5-13.5ukat/l


    Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own normal range. For diagnostic purposes, the alkaline phosphatase results should always be assessed in conjuction with the patients medical history, clinical examinations and other findings.

    Performance Characteristics


     With AutoPure Alkaline Phosphatase, the assay is linear upto 2000IU/I(33.4ukat/l). Determine samples with higher concentrations via the rerun function. On instruments without rerun function , manually dilute the samples with higher concentration using 0.9%NaCl or distilled/ deionized water  Multiply the result with the appropriate dilution factor


    There is no significant interference in samples containing upto 25 mg/dl of bilirubin and 400mg/dl of haemoglobin.


    Reproducibilty was determined using quality control sera as shown below


    Within run

    Between run

    Quality control material

    Mean IU/I

    SD IU/I


    Mean IU/I

    SD IU/I


    low control serum







    High control serum







    Trade Information
    • EXW ABPL
    • Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
    • 10000 Per Day
    • 2 Days
    • Yes
    • packaging : Empity
    • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
    • All India
    • certification : iso
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    Contact Us

    212, Udyog Mandir No. 17/C, Bhagoji Keer Marg, Mahim, Mumbai, Maharashtra, 400016, India
    Phone :+918045479256