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Alkaline Phosphatase 60

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Alkaline Phosphatase 60
Alkaline Phosphatase 60
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 1029 INR
    Product Specifications
    • Industrial
    • Industrial Lab Chemicals
    • 99%
    Product Description

    INTRODUCTION

    • Auto Zyme Alkaline Phosphatase is a reagent set for determination of Alkaline Phosphatase activity based on kinetic method using p-nitrophenyl phosphate (p-NPP).
    • Auto Zyme Alkaline Phosphatase is a single reagent system, using one step procedure.
    • Auto Zyme Alkaline Phosphatase has one step reconstitution. It involves dissolving Substrate tablet in Diluent.
    • Auto Zyme Alkaline Phosphatase is a High Stability Reagent.
    • Auto Zyme Alkaline Phosphatase is linear up to 700 IU/I.
    • Alkaline Phosphatase activity can be determined in just 21/2 minutes.
    • Auto Zyme Alkaline Phosphatase can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request.
    PRINCIPLE

    Alkaline Phosphatase cleaves p-nitrophenyl phosphate (p-NPP) into p-nitrophenol and phosphate. p-nitrophenol is a yellow colour compound in alkaline medium and absorbs light at 405 nm. The rate of increase in absorbance at 405 nm. is proportional to Alkaline phosphatase activity in specimen.

    PREPARATION OF  WORKING SOLUTION

    Reconstitute each substrate tablet with diluent as per the instruction indicated on the substrate bottle.

    REAGENT STORAGE & STABILITY

     

    • The reagent kit is stable till the expiry date stated on the label, when stored at 2 - 8 degree C.
    • The working solution is stable for 21 days at 2- 8 degree C.
    • The working solution should be prepared and stored in the dark (working solution bottle) provided. This is critical because the reagent is light sensitive. It should therefore be kept away from direct light.

    COMPONENTS & CONCENTRATION OF WORKING SOLUTION

     

    Component

    Concentration

    Diethanolamine buffer, pH 9.8

    1 moll

    p-nitrophenyl phosphate

    10 mmolll

    Magnesium chloride

    0.5 mmolll

    SPECIMEN COLLECTION & PRESERVATION

    Blood should be collected in a clean dry container. Haemolyzed specimen should be avoided as it may falsely elevate results. EDTA, Citrate and Oxalate inhibit Alkaline Phosphatase activity and should not be used as anticoagulant.

    For plasma separation any of the following two anticoagulants may be used :

     

    • HEPARIN 200 Uml blood
    • SODIUM FLUORIDE 10 mg/ml blood

     

    Serum / plasma should be separated immediately from cells. Alkaline Phosphatase is stable for 4 days at 2 - 8 degree C and several months when stored at -10 degreeC

    PROCEDURE
    • Reaction type: Kinetic
    • Reaction direction: Up
    • Wavelength: 405 nm.
    • Flowcell temperature: 37 degree C (+/- 0.2 degree C)
    • Zero setting with :Working Solution
    • Delay time:60 seconds
    • No. of readings :4
    • Interval: 30 seconds
    • Blank absorbance limit:  0.700 Abs.
    • Sample volume: 0.02 ml (20 ill )
    • Reagent volume: 1.0 ml
    • Factor: 2720
    • Linearity: 700 IU/1
    Manual assay procedure

    Prewarm the required amount of working solution at the required 

    Temperature 37 degree C before use. Perform the assay as given below :

    1.0 ml procedure

    Serum/Plasma

    0.02 ml (20 pi )

    Working Solution

    1.0 ml

    Mix thoroughly and transfer the assay mixture immediately to the thermo stated cuvette and start the stop watch simultaneously. Record the first reading at 60th second and subsequently, three more readings with 30 seconds interval at 405 nm.

    Conversion factors

    Following factors can be used for conversion of IU/1 from one temperature to another.

    Temperature Conversion 

    Temperature of

    Temperature factors

    assay

    25  degree C

    30 degree C

    37  degree C

    25  degree C

    1.0

    1.30

    1.80

    30 degree C

    0.75

    1.0

    1.35

    37  degree C

    0.55

    0.74

    1.0

     EXPECTED VALUES

     

    25 degree C

    30 degree C

    37 degree C

    ADULT

     

     

     

    (> 15 years) IU/1 CHILD

    60-170

    78-220

    108-306

    (< 15 years) IU/1

    150-450

    195-585

    210-810

     These expected values should be used as a guide only. It is recommended that each laboratory should establish its own expected values.

    PROCEDURE LIMITATIONS

     

    • The working solution is considered unsatisfactory and should not be used if the absorbance exceeds 0.700 at 405 nm. against distilled water.
    • If the A Abs./min exceeds 0.250, the specimen should be diluted 10 times (0.1 ml serum + 0.9 ml saline) and reassayed. Multiply the result obtained by 10.

     

    QUALITY CONTROL

    To ensure adequate quality control, it is recommended that each batch should include a normal and abnormal commercial reference control serum. It should be realised that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.

    Trade Information
    • EXW ABPL
    • Letter of Credit at Sight (Sight L/C), Letter of Credit (L/C), Cheque, Cash in Advance (CID), Cash Against Delivery (CAD)
    • 10000 Per Day
    • 2 Days
    • Yes
    • packaging : Empity
    • Africa, Middle East, Western Europe, Eastern Europe, South America, North America, Central America, Australia, Asia
    • All India
    • certification : iso
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    Phone :+918045479256