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Bilirunin (T And D) 200

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Bilirunin (T And D) 200
Bilirunin (T And D) 200
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 1283 INR
    Product Specifications
    • Industrial
    • Industrial Lab Chemicals
    • 95
    Product Description
    INTRODUCTION
    • Auto zyme Bilirubin is a reagent set for determination of Total and Direct Bilirubin based on Jurassic & Grof method, using Diazotized Sulphonic Acid with DMSO as an activator for Total Bilirubin.
    • Auto Zyme Bilirubin is a two reagent system using one step procedure.
    • Auto Zyme Bilirubin is linear upto 20 mg%.
    • Auto Zyme Bilirubin can be used on any Colorimeter, Spectrophotometer, Discrete semi-automated and Automated analyzer. Programmed can be designed for any specific analyzer upon request.
    • Auto Zyme Bilirubin has one step reconstitution.
    • Auto Zyme Bilirubin can be determined in just 5 minutes.
    • Auto Zyme Bilirubin method is relatively free of interference from hemoglobin and other commonly occurring substances in serum or plasma.
    • This insert covers technical matter for the complete range of Auto Zyme Bilirubin kits available viz.:

    Product

    Pack-Size

    Bilirubin T & D (Total and Direct) 200

    1 x 200 ml

    Bilirubin Total 1000

    1 x 1000 ml

    Bilirubin Direct 1000

    1 x 1000 ml

    Please read the appropriate matter depending on the kit you have purchased.

    PRINCIPLE

    Bilirubin reacts with diazotized sulphanilic acid to produce azobilirubin (violet colour). DMSO catalyzes the formation of azobilirubin from free bilirubin. The violet colour is proportional to bilirubin concentration measured at 546 nm.(530 - 550 nm.).

    TOTAL BILIRUBIN

    Bilirubin + Sulphanilic Acid + Sod. Nitrite DMSO x p Azobilirubin

    DIRECT BILIRUBIN

    Bilirubin + Sulphanilic Acid + Sod. Nitrite .Azobilirubin

    DMSO  Dimethyl Sulphoxide

    REAGENT STORAGE & STABILITY

    • The reagent kit should be stored at Room Temperature (25-30 degree C) and is stable till the expiry date indicated on the label.
    • The working solution is stable for 2 days at 2-8 degree C and for 5 hours at Room Temperature (25-30 degree C).
    COMPONENTS & CONCENTRATION OF WORKING SOLUTION

    Component

    Concentration

     

    Total Bilirubin

    Direct Bilirubin

    Sulphanilic Acid

    32 mmo1/1

    32 mmo1/1

    Sodium Nitrite

    290 mmo1/1

    29 mmo1/1

    Hydrochloric Acid

    165 mmo1/1

    165 mmo1/1

    DMSO

    7 mmo1/1

     

     SPECIMEN COLLECTION & PRESERVATION

    Blood should be collected in a clean dry container. Although serum is preferred plasma can also be used as sample.

    Following anticoagulant can be used for plasma separation :

    • EDTA 2 mg/ml of blood
    • HEPARIN 200 IU/ml of blood

    Bilirubin is light sensitive. Avoid exposure of serum or plasma to direct light. Bilirubin in serum and plasma is stable for a day at 2-8 degree C or one month at -10 degree C. Samples should be brought to room temperature before use.

    PROCEDURE

    • Reaction type: End-Point
    • Reaction time: 5 mins. at R.T. (25-30 degree C)
    • Wavelength: 546 nm.(530 - 550 nm.)
    • Zero setting with : Serum Blank
    • Sample volume: 0.05 ml (50 III)
    • Reagent volume: 1.02 ml
    • Factor: 20.2
    • Linearity: 20 mg%
    Manual assay procedure

    Perform the assay as given below :

    Serum Blank


    Test

    Reagent T1

     1.0 ml

    1.0 ml

    Reagent T2

     

    0.02 ml

    Serum / Plasma

    0.05 ml

    0.05 ml

     DIRECT BILIRUBIN

    Serum Blank

     

    Test

    Reagent T1

    1.0 ml

    1.0 ml

    Reagent T2

     

    0.02 ml

    Serum / Plasma

    0.05 ml

    0.05 ml

     Mix, and incubate the assay mixture at Room Temperature (25-30 degree C) for 5 minutes. Read before 8 minutes the absorbance of the test against their respective blanks at 546 nm (530 - 550 nm).

    EXPECTED VALUES

     

    • Total Bilirubin: up to 1.0 mg%
    • Direct Bilirubin: up to 0.3 mg%

     

    PROCEDURE LIMITATIONS

    Dilute the specimen if the Bilirubin value is above 20 mg%. Suitable dilution can be done with normal saline. In such case the results obtained should be multiplied by dilution factor to obtain correct Bilirubin value.

    QUALITY CONTROL

    It is recommended that each batch should include a normal and an abnormal commercial reference control serum or a known patient serum. Use of quality control serum, checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware, accuracy of pipetting and serum exposure to light.

    Trade Information
    • EXW ABPL
    • Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
    • 10000 Per Day
    • 2 Days
    • Yes
    • packaging : Empity
    • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
    • All India
    • certification : iso
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    Phone :08045479256