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BUN 100

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BUN 100
BUN 100
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 1046 INR
    Product Specifications
    • Biochemistry reagent
    • Liquid
    • Infinite Liquid BUN is aready-to-use, two liquid reagent system. Infinite Liquid BUN estimates urea nitrogen concentration in just 1 minute. Infinite Liquid BUN is linear upto 250 mg/dl.
    • other
    • 18 Months
    • Biochemistry reagent
    • Other
    • Other
    • Reagents
    • Other
    • ACC77889
    • Industrial
    • Industrial Lab Chemicals
    • 99%
      Product Description
      • Infinite Liquid BUN is a reagent set for determination of blood urea nitrogen (BUN) concentration in serum or plasma based on Enzymatic UV- Kinetic initial rate method.
      • Infinite Liquid BUN is aready-to-use, two liquid reagent system.
      • Infinite Liquid BUN estimates urea nitrogen concentration in just 1 minute.
      • Infinite Liquid BUN is linear upto 250 mg/dl. 
      • Infinite Liquid BUN can be used on any Spectrophotometer, Discrete Semiautomated and Automated analyzers. Programme can be designed for any specific analyzer upon request.
      • finite Liquid BUN is stable till expiry at 2 - 8C.
      PRINCIPLE

      Urea is hydrolysed to ammonia and carbondioxide by urease. Ammonia produced reacts with a-ketoglutarate to form glutamate in presence of glutamate dehydrogenase. NADH is oxidised to NAD in this reaction, which is measured as decrease in absorbance at 340 nm. The rate of decrease in absorbance at 340 nm. is directly proportional to BUN concentration in the specimen. 

      Urea + H,O- 2nH, + CO,

      GLDH

      NH, + a-ketoglutarate + NADH -glutamate + NAD

      Abbreviations

      GLDH - Glutamate dehydrogenase 

      REAGENT STORAGE & STABILITY

      • The reagent kit should be stored at 2 - 8 C and is stable till the expiry date indicated on the label. 
      • R, and R, reagents are stable till expiry at 2 - 8C.
      • The working solution (4 R, + 1 R,) is stable for 30 days at 2 - 8 C. 

      COMPONENTS & CONCENTRATION OF WORKING SOLUTION

      Component

      Concentration

      Tris buffer, pH 7.6

      120 mmol/l

      Urease

      20 KU/l

      Glutamate dehydrogenase

      1 KU/I

      NADH

      0.25 mmoll/|

      ketoglutarate

      10 mmol/l

       PROCEDURE

      • Reaction type :  UV-Kinetic 
      • Reaction direction:  Decreasing 
      • Wavelength :  340 nm 
      • Flowcell temperature:  .25 Degree C/30 Degree C
      • Zero setting with:  Distilled water 
      • Delay time:  300 seconds 
      • No. of readings:   2 
      • Interval :   30 seconds 
      • Sample volume:  1.000 Abs
      • R 1 volume:   360 Ul 
      • R 2 volume:  180 Ul 
      • Factor:   1743 
      • Linearity:   250mg /dl
      MANUAL ASSAY PROCEDURE .
      • Prewarm at 25Degree  C / 30 Degree  C the required amount of working solution before use. Perform the assay as given below : Working solution: 1.0 mi (800 U1 R, + 200 LI R,) Mix and aspirate. After the initial delay of 30 seconds, record the absorbance of the test at an interval of 30 seconds at 340 nm.
      • Determine the change in absorbance ( A Abs.) and calculate test result. 
      • Serum / Plasma

      Expected Values 

      • Urea Nitrogen (BUN) : 5-21 mg/dl
      • Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own normal range. 

      Trade Information
      • EXW ABPL
      • Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
      • 10000 Per Day
      • 2 Days
      • Yes
      • packaging : Empity
      • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
      • All India
      • certification : iso
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      Phone :+918045479256