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Calcium Arsenazo III 80

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Calcium Arsenazo III 80
Calcium Arsenazo III 80
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 1838 INR
    Product Specifications
    • other
    • Liquid
    • Other
    • AutoPure Calcium Arsenazo III is ready to use, single liquid reagent system. With AutoPure Calcium Arsenazo III, the assay is linear upto 15 mg/dl (3.75 mmo1/1).
    • Other
    • 18 Months
    • Biochemistry Reagent
    • Biochemistry Reagent
    • Reagents
    • Other
    • ACC9071
    • Industrial
    • Industrial Lab Chemicals
    • 99%
      Product Description

      Introduction

      • AutoPure Calcium Arsenazo III is a reagent kit for direct quantitative determination of calcium in human serum and plasma on automated clinical chemistry analyzers.
      • AutoPure Calcium Arsenazo III is ready to use, single liquid reagent system.
      • With AutoPure Calcium Arsenazo III, the assay is linear upto 15 mg/dl (3.75 mmo1/1).

      Principle

      • Arsenazo III reacts with calcium ions at neutral pH to form a blue coloured complex.
      • Ca + +Arsenazo III - Calcium - arsenazo complex 
      • The intensity of the blue colour formed is directly proportional to the concentration of calcium in the specimen and is measured photometrically.

      Reagent Storage, Stability & Handling 

       AutoPure Calcium Arsenazo III is ready to use, single liquid reagent system.

      Shelf life

      Stable till the expiry date indicated on the label, when stored at 15-25C.

      On - Board Reagent Stability

      • 45 days at 2- 8C after opening.
      • Protect the reagent from light and 
      • Do not freeze the reagent.

      Components & Concentration of Working Solution 

       Components                                                                                      Concentration

      • Imidazole buffer; pH 7.0                                                             100 mmol/l
      • Arsenazo III                                                                                90 umol/l
      • 8- Hydroxyquinoline                                                                    5 mmol/l
      • Stabilizers, excipients & surface active agents

      Specimen Collection & Preservation

      • Collect sample using standard sampling tube. Serum or heparinized plasma can be used. Do not use citrate, oxalate or EDTA as anticoagulants. Avoid venous stasis.
      • Calcium in serum/plasma is stable for 7 days at 20- 25C and for 21 days at 2 8C and 8 months at -20 C. Centrifuge samples containing precipitate before performing assay.

      Calculation

      • Fully automated system automatically calculates the Calcium concentration of each sample.
      • Results in mmo1/1= Results in mg/di x 0.25

      Application Sheet

      Refer to the application sheet for details. For additional system applications, contact our local Accurex representative. 

      Calibration

      For calibration it is recommended to use C.f.a.s or any other suitable calibrator material. 

      Calibration frequency

      Re  calibration is recommended

      • Whenever the reagent lot is changed.
      • As per the requirement of quality control procedures 

      Quality Control

      Each batch of AutoPure Calcium Arsenazo III is assayed with atleast six quality control sera- Precinorm  Precipath  Biorad 1 , Biorad 11 ,Accutrol Normal and Accutrol Abnormal prior to release.

      To ensure adequate quality control, it is recommended that the laboratory should use a normal and abnormal commercial reference control serum. It should be realized that the use of quality control material checks both the reagent and instrument functions together.

      If the control values fall outside the specified limits, each of the below criteria should be cross  checked and corrected:

      • Proper instrument function  wavelength setting, light source and temperature control. 
      •  Cleanliness of probes and cuvettes.
      • Bacterial contamination of wash water used by the instrument
      • Expiry date of the reagent kit. 

      Expected Values

      Serum/Plasma

      Adults : 8.6 -10.2 mg/dl (2.15 -2.55 mmol/l)

      Note:

      Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own normal range. For diagnostic purposes, the calcium results should always be assessed in conjunction with the patients medical history, clinical examinations and other findings.

      Performance Characteristics 

      Linearity

      With AutoPure Calcium Arsenazo III, the assay is linear upto 15 mg/dl (3.75 mmo1/1). Determine samples with higher concentrations via the rerun function. On instruments without rerun function, manually dilute samples with higher concentrations using 0.9% NaCI or distilled/deionized water (e.g. 1 + 2). Multiply the result by the appropriate dilution factor (e.g. 3). 

      Interference 

      There is no significant interference in samples containing upto 40 mg/dl of bilirubin and 1000 mg/dl of haemoglobin

      Precision 

      Reproducibility was determined using quality control sera as shown below:

       

      Within run

      Betweenrun

      Quality control marerial

      Means mg/dl

      SD mg/dl

      CV

      Means mg/dl

      SD mg/dl

      CV

      low control serum

      9.12

      0.09

      13.4

      9

      0.14

      1.5

      high control serum

      13

      0.1

      0.8

       

      0.14

      1

       Co   Relation Studies

      A comparison of the calcium determination using AutoPure Calcium Arsenazo III and AutoZyme Calcium Arsenazo III reagent gave the following co relation (mg/dl):

      Linear Regression

      • y = 0.949x+ 0.574
      • r= 0.941
      • Sy.x = 0.33

      No of samples measured : 72

      The sample concentrations measured were between 9 mg/dl and 12 mg/dl.

      References

      • Thomas L., Clinical Laboratory Diagnostics, 1st ed. Frankfurt : TH - Books Verlagsgesellschaft.,1998 :192 -202.
      • Endres, D.B., Rude, R.K., Mineral and Bone Metabolism. In : Burtis, CA., Ashwood, E.R., editors. Tietz Textbook of Clinical Chemistry, 3rd ed. philadelphia : W.B. Saunders Company ;1999 : 1395-1457.
      • Michaylova V., Ilkova, P. Photometric determination of micro amounts of calcium with arsenazo III. Anal. Chem. Acta, 1971; 53 : 194 -198.
      • Bauer, P.J. Affinity and Stoichiometry of calcium binding by arsenazo III. Anal. Biochem.1981; 110 : 61 - 72.
      Trade Information
      • EXW ABPL
      • Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
      • 10000 Per Day
      • 2 Days
      • Yes
      • packaging : Empity
      • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
      • All India
      • certification : iso
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