0
home

Chloride 100

home
Chloride 100
Chloride 100
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 802 INR
    Product Specifications
    • Biochemistry reagent
    • Biochemistry reagent
    • 18 Months
    • Other
    • Other
    • other
    • Reagents
    • Other
    • ACC74412
    • Industrial
    • Industrial Lab Chemicals
    • 99%
      Product Description
      INTRODUCTION
      • Auto Zyme Chloride is a reagent set for determination of chloride in serum/plasma based on Mercuric thiocyanate method.
      •  Auto Zyme Chloride is a single reagent system, ready-to-use.
      •  Auto Zyme Chloride estimates chloride in just 1 minute.
      •  Auto Zyme Chloride is linear up to 130 mmol/l.
      • Auto Zyme Chloride can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request.
      •  The shelf-life of Auto Zyme Chloride is 15 months
      PRINCIPLE

      Chloride reacts with mercuric thiocyanate to give mercuric chloride. The thiocyanate which is released reacts with ferric ions present in the reagent to form ferric thiocyanate which is directly proportional to chloride present in serum/plasma & can be measured at 510 nm.(500 - 520 nm.).

      PREPARATION OF WORKING SOLUTION

      The reagent is ready-to-use

      REAGENT STORAGE & STABILITY

      The reagents are for in vitro diagnostic use.

      Thiocyanate reagent and standard should be stored at temperature indicated on the bottle label.

      COMPONENTS & CONCENTRATION OF WORKING SOLUTION 

      Component

       Concentration

      Mercuric thiocyanate

       2.0 mmol/l

      Mercuric chloride

      0.8 mmol/l

      Ferric nitrate

       20.0 mmol/l

      Nitric acid

      45.0 mmol/l

      Stabilizers, surface active agent and inactive ingredients

       

       SPECIMEN COLLECTION & PRESERVATION

       

      • Blood should be collected in a clean dry container. Serum or plasma should be separated from cells as early as possible (within 30 minutes). Neatly separated
      • serum or plasma should be used.
      • Chloride is stable for 7 days in neatly separated serum/plasma at 2-8 degree C.

       

      PROCEDURE
      • Reaction type:  End Point
      • Reaction time:  1 min. at R.T. (25-30 degree C)
      • Wavelength : 510 nm.(500-520 nm.)
      • Zero setting with: Reagent Blank
      • Blank absorbance limit :   0.200 Abs.
      • Sample volume : 0.01 ml (10 ul)
      • Reagent volume:  1.0 ml
      • Standard concentration:  100 mmol/l
      • Linearity: 130 mmol/l
      Manual assay procedure

      Perform the assay as given below :

      1.0 ml procedure

       

      Serum/Plasma

      Standard

      Blank

      Thiocyanate Reagent

      0.01 ml

      0.01 ml

       

      1.0 ml

      1.0 ml

      1.0 ml

       Incubation

      Incubate the assay mixture for 1 minute at room temperature (25-30 degree C). After completion of incubation period measure the absorbance of assay mixture against blank at 510 nm. The final colour is stable for one hour if not exposed to direct light.

      EXPECTED VALUES

      98 - 107 mmol/l 

      PROCEDURE LIMITATIONS

       

      • Discard the working reagent if its absorbance is more than 0.200 against distilled water at 510 nm.
      •  Strong lipemic and haemolytic sera should not be used.
      •  Contaminated glassware is the greatest source of error. Disposable plastic wares are recommended for the test.
      • The reagent contains nitric acid. Avoid contact with skin and mucous membrane. If you come in contact with the reagent wash thoroughly with water.
      • The use of normal saline should be avoided. It may contaminate the reagent and lead to erroneous results 

       

      QUALITY CONTROL

      To ensure adequate quality control, it is recommended that each assay should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both, instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, cleanliness of glassware and accuracy of pipetting.
      Trade Information
      • EXW ABPL
      • Paypal, Cash Against Delivery (CAD), Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C), Cheque, Cash in Advance (CID), Cash Advance (CA)
      • 10000 Per Day
      • 2 Days
      • Yes
      • packaging : Empty
      • Africa, Middle East, Western Europe, Eastern Europe, South America, North America, Central America, Australia, Asia
      • All India
      • certification : iso
      Related Products
      LDL Cholesterol 240
















      Send Inquiry
      Autopure  HbA1c Control Low And High 1
















      Send Inquiry
      Phosphorus 100
















      Send Inquiry
      Triglycerides 600 Infinite
















      Send Inquiry
      LDH 48
















      Send Inquiry
      Magnesium 50
















      Send Inquiry
      right arrow
      left arrow
      Contact Us

      212, Udyog Mandir No. 17/C, Bhagoji Keer Marg, Mahim, Mumbai, Maharashtra, 400016, India
      Phone :+918045479256