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CK MB 30

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CK MB 30
CK MB 30
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 5644 INR
    Product Specifications
    • 18 Months
    • Biochemistry reagent
    • Liquid
    • other
    • Infinite Liquid CK - MB is a ready-to-use, two liquid reagent system. Infinite Liquid CK - MB estimates CK - MB activity in just 6/ minutes. Infinite Liquid CK - MB is linear upto 2000 IU/I.
    • Other
    • Other
    • Reagents
    • Other
    • ACC171145
    • Industrial
    • Industrial Lab Chemicals
    • 99%
    Product Description
    INTRODUCTION
    • Infinite Liquid CK - MB is a reagent set for determination of CK - MB activity in serum and plasma based on immunoinhibition method.
    • Infinite Liquid CK - MB is a ready-to-use, two liquid reagent system.
    • Infinite Liquid CK - MB estimates CK - MB activity in just 6/ minutes.
    • Infinite Liquid CK - MB is linear upto 2000 IU/I.
    • Infinite Liquid CK - MB can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzers. Programme can be designed for any specific analyzer upon request.
    • Infinite Liquid CK - MB is stable till expiry at 2 - 8 Degree C.
    PRINCIPLE

    The serum sample is incubated with CK - MB reagent containing antibody specific to CK-M subunit which completely inhibits the CK-M monomer. The activity of CK-B which is not inhibited by the antibody, is then measured by the following reaction sequence:

    Abbreviations

    • ADP: Adenosine-5-diphosphate
    • ATP: Adenosine-5-triphosphate
    • G-6-PDH: Glucose-6-phosphate dehydrogenase 
    • HK: Hexokinase
    PREPARATION OF WORKING SOLUTION
    • R1 : Ready-to-use reagent. 
    • R2 : Ready-to-use reagent.
    • Prepare working solution by mixing Reagent R1 and Reagent R2 in the ratio 4 : 1 as per requirement.
    REAGENT STORAGE & STABILITY
    • The reagent kit should be stored at 2 - 8 Degree C and is stable till the expiry date indicated on the label.
    • R1 & R2 reagents are stable till expiry at 2 - 8 Degree C.
    • The working solution (4 R1 + 1 R2) is stable for 10 days at 2 - 8 Degree C.

    COMPONENTS & CONCENTRATION OF WORKING SOLUTION

    Component

    Concentration

    Component

    Concentration

    Imidazole buffer; pH 6.7

    100 mmol/I

    EDTA

    2 mmol/I

    Phosphocreatine

    30 mmol/I

    ADP

    2 mmol/I

    N-acetylcysteine (NAC)

    20 mmol/I

    NADP

    2 mmol/I

    Magnesium acetate

    10 mmol/I

    AMP

    5 mmolJ

    Glucose

    20 mmol/I

     

     


    Di-(adenosine-5)-pentaphosphate

    10 Mmol/I

    Glucose-6-phosphate dehydrogenase

    1.5 KU/I

    Hexokinase

     2.5 KU/I


    SPECIMEN COLLECTION & PRESERVATION

    Blood should be collected in a clean dry container Although serum is preferred, plasma with heparin or EDTA can be used. The assay should be carried out on the same day as far as possible. Serum and plasma samples are stable for 3 days at 2 - 8 Degree C and 1 month at -20 Degree C. The samples should be brought to room temperature prior to use. Avoid use of haemolysed and grossly contaminated samples

    PROCEDURE

    • Reaction type: UV - Kinetic
    • Reaction direction: Increasing
    • Wavelength: 340 nm.
    • Flowcell  temperature:37 Degree C
    • Zero setting with: Distilled water
    • Delay time : 300 seconds
    • No. of readings: 4
    • Interval: 30 seconds
    • Sample volume: 0.05 ml (50 |tl)
    • Working solution volume (4R, : 1R ): 1.0 ml (1000 |Jl) Factor 6752
    • Linearity: 2000 IU/I 
    Manual assay procedure

    • Prewarm at 37 Degree C the required amount of working solution be- fore use. Perform the assay as given below :
    • 1.0 ml procedure 
    • Serum / plasma: 0.05 ml
    • Working solution: 1.0 ml (800 Al R1 + 200 [II  R2)

    Mix and aspirate. After the initial delay of 390 seconds, record the absorbance of the test at an interval of 30 seconds  for the next 90 seconds at 340nm. Determine the mean change in absorbance and calculate the test results.

    Calculation

    Activity of CK - MB in IU/I -A Abs./min. x 6752

    Temperature Conversion 

    Assay temperature

    Desired temperature

    25 C

    30 Degree C

    37 Degree C

    25 Degree C

    1.00

    1.53

    2.38

    30 Degree C

    0.65

    1.00

    1.56

    37 Degree C

    0.42

    0.64

    1.00

     EXPECTED VALUES

    Temperature

    at 25 Degree C

    at 30 Degree C

    at 37 Degree C

    CK-MB

    10 IU/I

     15 IU/I

    24 IU/I

    Trade Information
    • EXW ABPL
    • Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
    • 10000 Per Day
    • 2 Days
    • Yes
    • packaging : Empity
    • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
    • All India
    • certification : iso
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    Phone :+918045479256