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CK NAC 30

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CK NAC 30
CK NAC 30
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 2791 INR
    Product Specifications
    • Other
    • Cool & Dry Place
    • other
    • Liquid CK - NAC is linear upto 2000 IU/I. Liquid CK - NAC can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzers. Programme can be designed for any specific analyzer upon request.
    • Powder
    • Other
    • 18 Months
    • Cool & Dry Place
    • Reagents
    • Other
    • ACC66452
    • Industrial
    • Industrial Lab Chemicals
    • 99%
      Product Description
      INTRODUCTION
      • Liquid CK - NAC is a reagent set for determination of creatine kinase activity in serum and plasma based on UV - Kinetic method.
      • Liquid CK - NAC is a ready-to-use, two liquid reagent system.
      • Liquid CK NAC estimates creatine kinase activity in just 4/ minutes.
      •  Liquid CK - NAC is linear upto 2000 IU/I.
      • Liquid CK - NAC can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzers.  Programme can be designed for any specific analyzer upon request.
      • Liquid CK NAC is stable till expiry at 2 8 Degree C
      PRINCIPLE

      Creatine kinase catalyzes the conversion of creatine phosphate and ADP to creatine and ATP. ATP phosphorylates glucose to glucose-6-phosphate in the pre sence of hexo kinase. GIucose-6-ph osp hate is ox idized to 6-phosphogIuconate, reducing NADP to NADPH in presence of G-6-PDH. The rate of increase in NADPH absorbance at 340 nm. is directly proportional to the activity of creatine kinase in serum/plasma.

      Abbreviations

      • ADP: Adenosine - 5 diphosphate
      • ATP: Adenosine - 5 triphosphate
      • G-6-PDH: Glucose-6-phosphate dehydrogenase CK Creatine kinase
      • HK: Hexokinase

       PREPARATION OF WORKING SOLUTION

      Prepare working solution by mixing Reagent R, and Reagent R2 in the ratio 4 : 1 as per requirement.

      REAGENT STORAGE & STABILITY

      The reagent kit should be stored at 2 8 C and is stable till the expiry date indicated on the label.

      The working solution (4 R1 + 1 R2) is stable for 10 days at 2 - 8 Degree C.

      COMPONENTS & CONCENTRATION OF WORKING SOLUTION 

      Component

      Concentration

      Component

      Concentration

      Imidazole buffer pH 6.7

      100 mmol/I

      EDTA

      2 mmol/I

      Phosphocreatine

      30 mmol/I

      ADP

      2 mmol/I

      N-acetylcysteine (NAC

      20 mmol/I

       NADP

      2 mmol/I

      Magnesium acetate

      10 mmol/I

      AMP

      5 mmol/I

      Glucose

      20 mmol/I

       

       

      Di-(adenosine-5)-pentaphosphate

      10 mmol/I

      Glucose-6-phosphate dehydrogenase

      1  1.5 KU/I

      Hexokinase

      1  2.5 KU/I

      SPECIMEN COLLECTION & PRESERVATION

      Blood should be collected in a clean dry container. Although serum is preferred, plasma with heparin or EDTA can be used. The assay should be carried out on the same day, as far as possible. Serum and plasma samples are stable for 1 week at 4 C and 1 month at -10 C. The samples should be brought to room temperature prior to use. Avoid use of haemolysed and grossly contaminated samples.

      PROCEDURE

      • Reaction type: UV - Kinetic
      • Reaction direction: Increasing
      • Wavelength: 340 nm.
      • Flowcell  temperature: 37 Degree C
      • Zero  setting with:  Distilled water
      • Delay  time :  180 seconds
      • No. of readings: 4
      • Interval:  30 seconds
      • Sample  volume: 0.04 ml (40 II)
      • Working solution volume:  (4R  1R2)1.0 ml (1000 II)
      • Factor: 4127
      • Linearity:  2000 IU/I 
      Manual assay procedure

      Prewarm at 37 Degree C the required amount of working solution be- fore use. Perform the assay as given below :

      1.0 ml procedure
      • Serum / plasma 0.04 ml
      • Working solution : 1.0 ml (800 JI R1 + 200 II R2)
      Mix and aspirate. After the initial delay of 180 seconds, record the absorbance of the test at an interval of 30 seconds for the next 9 0 Second s at 340 nm. Determine the mean change in absorbance per minute and calculate test results.

      Calculation:

      Calculate the  change  in  absorbance  per  minute. ( A Abs./30 seconds x 2)

      Activity of CK - NAC in IU/I - A Abs./min x 4127

      Temperature Conversion 

      Assay temperature

      Desired temperature

      25 C

      30 Degree C

      37 Degree C

      25 Degree C

      1.00

      1.53

      2.38

      30 Degree C

      0.65

      1.00

      1.56

      37 Degree C

      0.42

      0.64

      1.00

      EXPECTED VALUES

      Temperature

      at 25 Degree C

      at 30 Degree C

      at 37 Degree C

      MEN

      80 IU/I

      130IU/I

      190IU/I

      WOMEN

      70 IU/I

      110IU/I

      165IU/I

      BABIES (2-12 months)

      136 IU/I

      210IU/I

      325 IU/I

      CHILDREN (above 12 months)

      94 IU/I

      150 IU/I

      225 IU/I


      QUALITY CONTROL

      To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.

      Trade Information
      • EXW ABPL
      • Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
      • 10000 Per Day
      • 2 Days
      • Yes
      • packaging : Empity
      • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
      • All India
      • certification : iso
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      212, Udyog Mandir No. 17/C, Bhagoji Keer Marg, Mahim, Mumbai, Maharashtra, 400016, India
      Phone :+918045479256