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Creatinine 200

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Creatinine 200
Creatinine 200
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 1365 INR
    Product Specifications
    • Biochemistry reagent
    • other
    • Biochemistry reagent
    • Other
    • AutoPure Creatinine is a reagent kit for direct quantitative determination of Creatinine in human serum and urine based on enzymatic method.
    • 18 Months
    • Other
    • Liquid
    • Reagents
    • Other
    • ACC6332
    • Industrial
    • Industrial Lab Chemicals
    • 99%
      Product Description

      Introduction

      •  AutoPure Creatinine  is a reagent kit for direct quantitative determination of  Creatinine in human serum and urine based on enzymatic method.
      •   AutoPure Creatinine is ready to use , two  liquid reagent system.
      • With   AutoPure Creatinine, the assay is linear upto  200 mg/dl.

      Clinical Significance

      Creatinine is a chemical waste molecule that is generated from muscle metabolism. Creatinine is produced from Creatinine, a molecule of major importance for energy production in muscles. Approximately 2 % of the bodys Creatinine is converted to Creatinine every day.

      Creatinine is transported through the bloodstream to the kidneys. The kidneys filter out most of the Creatinine and dispose . It of in the urine. The kidneys maintain the blood Creatinine in a normal range.

      Creatinine has been found to be a fairly reliable indicator of kidney function. As the kidney become impaired the Creatinine level in the blood will rise. Abnormally high levels of Creatinine thus warn of posssible malfunction or failure of the kidneys.

      Principle 

      Through a series of enzymatic reactions,  Creatinine is converted to glycine. Endogenous components such as creatine and sarcosine are eliminated in the first step of the reaction. The hydrogen peroxide formed reacts with TOOS  in the presence of peroxidase ( POD), to form a quinone dye.

      The intensity of the colour is directly proportional to the concentration of Creatinin in the specimen and is measured photometrically at 546 nm.

      • Creatinine + H2O- _Creatinine
      • Creatinine + H20- Sarcosine + Urea 
      • Sarcosine + 02+ H20 - Glyine + HCH0+ H2O2
      • 2H202+ 4- AAP+ TOOS - Quinoone dye + 4 H20
      • TOOS : N-ethyl-N-( 2-hydroxy-3 sulfopropyl)- m- toluidlne 
      • 4-AAP: 4 Aminoantipyrine

      Reagent Storage, Stability & Handling

      AutoPure  Creatinine is a ready to use two liquid reagent system.   

      Shelf life  

      Stable till the expiry date indicated on the label, when stored at 2-8 C. 

      On Board Reagent Stability 

      •  R1: 28 days at 2- 8 C after opening
      • R2: 28 days at 2- 8 C after opening

      Specimen collection & Preservation

      • Collect sample using standard sampling tube. Serum is the specimen of choice. 
      • Urine samples ( 24 hrs) should be diluted appropriately before use.
      • Creatinine in samples is stable for 24 hours if stored at 2 - 8 C .

      Calculations 

      Fully automated system automatically calculated the Creatinine   concentration of each samples.

      Calibration

      Re- calibration is recommended 

      • Whenever the reagent lot is changed .
      • As per the requirement of quality control procedured.

      Quality Control

      Each batch of autopure Creatinine     is assayed with atleast six quality control sera- Precinorm, precipath, bioradl, bioradll, accutrol normal and Accutrol abnormal prior to release.

      To ensure adequate quality control, it is recommended that the laboratory should use a normal and abnormal commerical reference control serum. It should be realized that the use of quality control material checks both the reagent and instrument functions together.

      If the control values fall outside the specified limits, each of the below criteria should be cross checked and corrected:

      • Proper instruments function- wavelength setting, light source and temperature control.
      • Cleanliness of probes and cuvettes.
      • Bacterial contamination of wash water used by the instrument.
      • Expiry date of the reagent kit. 

      Expected Values 

       

      Serum

      Urine

      Men

      0.7-1.2 mg/Dl

      40- 278 mg/Dl

      Women

      0.5-1.0 mg/Dl

      29-226 mg/Dl

      Expected range varies from population to population. it is therefore recommended that each laboratory should establish its own normal range. For diagnostic purposes, the bilirubin results should always be assessed in diagnostic purposes, the bilirubin results should always be assessed in conjuction with the patients medical history clinical examinations and other findings.

      Performance Characteristics 

      Linearity 

      With Autopure Creatinine   , the assay is linear up to200mg/dL(458.9 mmol/l) .Determine samples with higher concentration via the rerun function . On instrument without rerun function, manually dilute samples with higher concentration using  normal saline (0.9% Nacl) or distilled/deionized water and repeat the assay. The result obtained should be then  multiplied with dilution factor to obtain correct creatinine concentration.. 

      Interference  

      There is no significant interference in samples containing upto 1000 mg/dl Triglyceride and 40mg/dl Bilirubin.

      Precision 

       Reproducibility was determined using three level  control sera specimen as shown below 

       

      Within run (n=80)

      Between run (n=80)

      Serum

      Mean ( mg/Dl)

      SD (mg/Dl)

      CV

      Mean ( mg/Dl)

      SD (mg/Dl)

      CV

      Sample 1

      0.74

      0.015

      2.1

      0.74

      0.022

      3.0

      Sample 2

      1.38

      0.015

      1.1

      1.38

      0.026

      1.9

      Sample 3

      4.04

      0.029

      0.7

      4.04

      0.058

      1.4

       Co- Relation Studies

      A comparison of the Creatinine   determination using autopure Creatinine   and reference competitors product  gave the following co- realtion (mg/dL):

      Linear Regression (sERUM):

      • y=      0.9467 x + 0.0643
      • r2=    0.9981
      • Sy.x=   1.46

      No of samples measured : 55

      Trade Information
      • EXW ABPL
      • Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
      • 10000 Per Day
      • 2 Days
      • Yes
      • packaging : Empity
      • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
      • All India
      • certification : iso
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      212, Udyog Mandir No. 17/C, Bhagoji Keer Marg, Mahim, Mumbai, Maharashtra, 400016, India
      Phone :+918045479256