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Diluent Glucose STAT 2000
- Kit/Kits
- 1
- Kit/Kits
- 3436 INR
- Biochemistry Reagent
- Biochemistry Reagent
- Auto Zyme STAT Glucose pack sizes 10 x 100 ml and 4 x 500 ml are provided with 2 standards of 100 mg% and 300 mg%. Auto Zyme STAT Glucose is Linear up to 700 mg% glucose for kinetic assay procedure and 500 mg% using end-point procedure. Auto Zyme STAT Glucose is a High Stability Reagent.
- 12 Months
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- Other
- Liquid
- Other
- Reagents
- Other
- ACC129963
- Industrial
- Industrial Lab Chemicals
- 99%
- Auto Zyme STAT Glucose is a reagent set for determination of True Glucose based on enzymatic method using Glucose oxidase and Peroxidase.
- Auto Zyme STAT Glucose estimates glucose in just one minute using an initial rate method and in 7 minutes by end-point method.
- Auto Zyme STAT Glucose pack sizes 10 x 100 ml and 4 x 500 ml are provided with 2 standards of 100 mg% and 300 mg%.
- Auto Zyme STAT Glucose is Linear up to 700 mg% glucose for kinetic assay procedure and 500 mg% using end-point procedure.
- Auto Zyme STAT Glucose is a High Stability Reagent.
- Auto Zyme STAT Glucose can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request.
- Auto Zyme STAT Glucose has one step reconstitution. It involves the mixing of ENZYME and DILUENT.
- Sodium Fluoride (as an anticoagulant up to 10 mg/ml blood) does not effect glucose assay.
- The influence of Ascorbate, Bilirubin, Antidiabetic drugs and Hemoglobin is negligible.
- Very small volume of serum or plasma is required for assay.
- The shelf-life of Auto Zyme STAT Glucose is 18 months.
Glucose oxidase (GOD) converts Glucose to gluconic acid. Hydrogen peroxide formed in this reaction, in presence of Peroxidase (POD), oxidatively couples with 4-aminoantipyrine / phenol to produce red quinonimine dye. This dye has absorbance maximum at 505 nm. (500-550 nm.). The intensity of the colour complex is directly proportional to the Glucose in specimen.
REAGENT AVAILABILITY
ENZYME Vials | 10 x 100 ml | 4 x 500 ml (Code GU-3) 4 |
STANDARD bottle -100 mg% | 1 | 1 |
STANDARD bottle - 300 mg% | 1 | 1 |
WORKING Solution Container* | 1 |
|
DILUENT bottles | 2 | 4 |
Empty bottle for preparation and storage of working solution)
GLUCOSE standard 100 mg% and 300 mg% are assayed against National Bureau of Standards (NBS) reference material from Washington.
ENZYME and STANDARD are to be stored at 2-8 degree C.
Diluent Reagent may be stored below 30 degree C and away from direct light. The reagents are for in vitro diagnostic use.
PREPARATION OF WORKING SOLUTION
- 4 x 500 ml (Code GU-3)
- Transfer the content of one vial of Enzyme into one bottle of Diluent and mix by gentle swirling or inversion. Do not shake vigorously.
- 10 X 100 ml (Code GU-2)
- Transfer 100 mL. Diluent into working solution bottle. Now add contents of one vial of Enzyme into it. Mix by gentle swirling or inversion. Do not shake vigorously.
- Stability of reconstituted working reagent is printed on the bottle label.
- (DO NOT FREEZE). The working solution should be stored in the dark bottle (diluent bottle or the working solution bottle) provided. This is critical because the reagent is light sensitive (auto oxidation of chromogen system by light and air), it should therefore be kept away from direct light.
COMPONENTS & CONCENTRATION OF WORKING SOLUTION
Component | Concentration |
Phosphate Buffer pH 7.0 | 170 mmo1/1 |
Glucose oxidase | 15000 IU/1 |
Peroxidase | 1500 IU/1 |
4-aminoantipyrine | 0.28 mmo1/1 |
Phenol | 16 mmo1/1 |
SPECIMEN COLLECTION & PRESERVATION
Blood should be collected in a clean dry container. Serum or plasma should be separated from the cells at the earliest possible (within 30 minutes), as the rate of glycolysis is approximately 7 mg% per hour at room temperature.
For plasma separation following anticoagulants may be used.
- EDTA 2 mg/ml of blood
- CITRATE 6 mg/ml of blood
- HEPARIN 200 Wml of blood
- OXALATE 3 mg/ml of blood
- SODIUM FLUORIDE 10 mg/ml of blood
Sodium Fluoride is preferred as anticoagulant due to its antiglycolytic activity. The higher concentration of Sodium fluoride i.e. more than 10 mg/ml blood should be avoided as it may inhibit the colour development. Glucose is stable for 24 hours in neatly separated plasma and serum. If the estimation is not possible within 24 hours then the specimen should be preserved at -10 degree C and should be used within 30 days.
PROCEDURE FOR END-POINT
- Reaction type: End-Point
- Reaction time: 7 mins. at 37 degree C/15 mins. at R.T. (25-30 degree C)
- Wavelength: 505 nm. (500-550 nm.)
- Zero setting with: Reagent Blank
- Blank absorbance limit: 0.300 Abs.
- Sample volume: 0.01 ml (100)
- Reagent volume: 1.0 ml
- Standard concentration: 100 mg%
- Linearity: 500 mg/dl
1.0 ml procedure
| Serum / Plasma | Standard | Blank |
Working Solution | 0.01 ml | 0.01 ml |
|
1.0 ml | 1.0 ml | 1.0 ml |
Incubation
Incubate the assay mixture for 7 minutes at 37 degree C or 15 minutes at room temperature (25 - 30 degree C). After completion of incubation period measure the absorbance against blank at 505 nm. Final colour is stable for at least two hours if not exposed to direct light.
PROCEDURE FOR KINETIC ASSAY
- Reaction type:2 Point-Kinetic
- Reaction direction : Upward
- Wavelength : 505 nm. (500 - 550 nm.)
- Flow cell temperature :37 degree C
- Zero setting with : Reagent Blank
- Delay time : 20 secs.
- No. of readings: 2
- Interval: 40 secs.
- Blank absorbance limit: 0.300 Abs.
- Sample volume: 0.01 ml (10 gl)
- Reagent volume: 1.0 ml
- Factor: 100 (A Abs. of std.)
- Linearity: 700 mg/dl
Perform the assay as given below :
1.0 ml procedure
- Standard/Specimen : 0.01 ml (10 ill)
- Working solution: 1.0 ml
First carry out the assay of standard.
Mix and start stop watch simultaneously. Record absorbance at exactly 20th second after standard addition and then again at 60 degree second.
Subsequently, carry out the assay of the specimen following exactly the same procedure mentioned above.
EXPECTED VALUES
- Fasting Blood Glucose : 60 to 110 mg%
- Postprandial Blood Glucose : 145 mg%
- Discard the working solution if the absorbance is more than 0.300 against distilled water at 505 nm.
- If the glucose value exceeds linearity limit then dilute specimen suitably with normal saline and repeat the assay. In such case the assay value should be multiplied with the dilution factor to obtain correct glucose value of the specimen.
- EXW ABPL
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- 10000 Per Day
- 2 Days
- Yes
- packaging : Empity
- Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
- All India
- certification : iso
212, Udyog Mandir No. 17/C, Bhagoji Keer Marg, Mahim, Mumbai, Maharashtra, 400016, India
Phone :+918045479256