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G6PDH Quantitative Kinetic 12

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G6PDH Quantitative Kinetic 12
G6PDH Quantitative Kinetic 12
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 1960 INR
    Product Specifications
    • Other
    • Auto Zyme NEW G6PDH is a reagent set for quantitative determination of G6PDH activity in erythrocytes by kinetic method. Auto Zyme NEW G6PDH can be used on any semi-automated analyzer.
    • Liquid
    • Biochemistry reagent
    • 18 Months
    • Other
    • other
    • Biochemistry reagent
    • Reagents
    • Electrolyte
    • ACC3341
    • Industrial
    • Industrial Lab Chemicals
    • 99%
      Product Description
      INTRODUCTION
      • Auto Zyme NEW G6PDH is a reagent set for quantitative determination of G6PDH activity in erythrocytes by kinetic method.
      • Auto Zyme NEW G6PDH can be used on any semi-automated analyzer.
      PRINCIPLE

      G6PDH present in the erythrocytes (RBCs) is extracted by lysing the cells using lysing reagent. The extracted enzyme catalyzes the following reaction :

      G6PDH

      Glucose-6-Phosphate + NADP P 6-Phosphogluconate + NADPH

      The conversion of co-enzyme NADP to NADPH is proportional to the concentration of G6PDH activity in the sample and is measured at 340 nm as rate of increase in absorbance.

      PREPARATION OF WORKING SOLUTION
      • Reconstitute one vial of R1 with 1.1 ml of R2 to prepare working solution. Mix by gentle swirling or inversion. Do not shake vigorously.
      • G6PDH R3 is ready-to-use and should be used pre-cooled at 2 - 8 degree C.
      REAGENT STORAGE & STABILITY
      • The working solution is stable for 7 days at 2 - 8 degree C.
      • The reagent kit should be stored at 2 - 8 degree C and is stable till the expiry date indicated on the label.

      REAGENT COMPONENTS

      R1

       Co-enzyme/Substrate

      12 vials

      R2

      Buffer

      1 bottle

      R3

      Lysing Reagent

      1 bottle

       SPECIMEN COLLECTION  & PRESERVATION

       

      • Whole blood should be collected in a clean and dry container with citrate or oxalate.
      • Determine the Hemoglobin content of the whole blood or the RBC count prior to lysis of the cells.

       

      PROCEDURE
      • Reaction type: Kinetic
      • Reaction direction : Increasing
      • Wavelength: 340 nm
      • Flowcell temp: 30 degree C/37 degree C
      • Zero setting with :Distilled water
      • Delay time: 180 seconds
      • No. of readings: 4
      • Interval: 60 seconds
      • Sample volume: 0.025 ml (25 NI)
      • Working solution volume:1.0 ml (1000 NI) 
      STEP I : PREPARATION OF RED CELL HAEMOLYSATE

      Wash 0.1 ml of whole blood with 2 ml of physiological saline (0.9% NaCI) 3 times and suspend the washed, packed and centrifuged erythrocytes in pre-cooled 0.5 ml of G6PDH R3 (Lysing Reagent). Mix well and keep in the refrigerator (2 - 8 degree C ) for at least 15 minutes and maximum for 2 hours. Centrifuge the lysate at 3000 r.p.m. for 5 minutes prior to use as given below:

      STEP II : MANUAL ASSAY PROCEDURE

      Reagents

      Test

      Working Solution

      1.0 ml

      Hemolysate

      25 NI

      Mix and aspirate. After the initial delay of 180 seconds, record the absorbance of the test at an interval of one minute for the next 3 minutes at 340 nm. Determine the mean change in absorbance per minute and calculate test results.

      EXPECTED VALUES

       

      • G6PDH Activity (at 30 degree C) : 146 - 376 U/1012RBCs : 4.6 - 13.5 U/g Hb
      • (at 37 degree C) : 203 - 523 U/1012 RBCs : 6.4 - 18.8 U/g Hb

       

      QUALITY CONTROL

      To ensure adequate quality control, it is recommended that each batch should include normal and abnormal reference control. It should be realised that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.

      Trade Information
      • EXW ABPL
      • Paypal, Letter of Credit at Sight (Sight L/C), Letter of Credit (L/C), Cheque, Cash in Advance (CID), Cash Against Delivery (CAD)
      • 10000 Per Day
      • 2 Days
      • Yes
      • packaging : Empity
      • Africa, Middle East, Western Europe, Eastern Europe, South America, North America, Central America, Australia, Asia
      • All India
      • certification : iso
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      Phone :+918045479256