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Gamma-GT 60

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Gamma-GT 60
Gamma-GT 60
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 5673 INR
    Product Specifications
    • Other
    • AutoPure Gamma- GT is a reagent kit for direct gamma - glutamyltransferase ( GGT ) in human serum and plasma on automated clinical chemistry analyzers. AutoPure Gamma- GT is ready to use , two liquid reagent system.
    • other
    • Biochemistry Reagent
    • Liquid
    • Other
    • 18 Months
    • Biochemistry Reagent
    • Reagents
    • Other
    • ACC9052
    • Industrial
    • Industrial Lab Chemicals
    • 99%
      Product Description

      Introduction

      •  AutoPure Gamma- GT  is a reagent kit for direct gamma - glutamyltransferase ( GGT ) in human serum and plasma on automated clinical chemistry analyzers.
      •   AutoPure Gamma- GT is ready to use , two  liquid reagent system.
      • With   AutoPureGamma- GT the assay is linear upto  1200 IU/I( 20.04 ukat/l)).

      Reagent Storage, Stability & Handling

      AutoPureGamma- GT is a ready to use two liquid reagent system.   

      Shelf life  

      Stable till the expiry date indicated on the label, when stored at 2- 8 C.  

      On Board Reagent Stability

      • R1 : 21 days at 2- 8 C after opening. Protect the reagent from light and contamination.
      • R2:  21 days at 2- 8 C after opening. Protect the reagent from light and contamination.

      Do not freeze the reagent.

      Note : Over a period of time, the reagent R2 may develop a yellow colour. This is expected and does not impair the performance of the assay.

      Components & Concentration of Working Solution 

      Components                                                                              Concentration

      R1

      • Glycylglycine : pH 8.5                                                                 200 mmol/l
      • Stabilizers excipients & surface active agents 

      R2

      • MES buffer : pH5.5                                                                            20 mmol/l
      • L-y-glutamyl-3-carboxy-4-nitroanilide                                                >25 mmol/l
      • Stabilizers excipients & surface active agents 

      Specimen collection & Preservation

      • Collect sample using standard sampling tube. Although Serum is preferred  heparinized plasma or EDTA   plasma can be used.
      • GGT  in serum/ plasma is stable for 7 days at 2- 8 C and 180days at -20 C. Centrifuge samples containing pricipate before performing assay.

      Calculations 

      • Fully automated system automatically calculated the GGT  activity of each samples. 
      • Result in mkat/l = Result in IUI x 0.016 7

      Application Sheet 

      Application sheet for the popular fully automated are provided along with the kit. For additional system applications, contact our local accurex representative. 

      Calibration

       Determine the factor using calibrator.For  calibration it is recommended  to use C.f. a. s or any other suitable calibrator material. 

      Calibration

      Re- calibration is recommended 

      • Whenever the reagent lot is changed .
      • As per the requirement of quality control procedured.

      Quality Control

      Each batch of autopure Gamma-GT   is assayed with atleast six quality control sera- Precinorm, precipath, bioradl, bioradll, accutrol normal and Accutrol abnormal prior to release.

      To ensure adequate quality control, it is recommended that the laboratory should use a normal and abnormal commerical reference control serum. It should be realized that the use of quality control material checks both the reagent and instrument functions together.

      If the control values fall outside the specified limits, each of the below criteria should be cross checked and corrected:

      • Proper instruments function- wavelength setting, light source and temperature control.
      • Cleanliness of probes and cuvettes.
      • Bacterial contamination of wash water used by the instrument.
      • Expiry date of the reagent kit. 

      Expected Values 

      Serum/Plasma

      IU/I

      Men

      8-61

      Women

      5-36

      ukat/l

      Men

      0.13-1.02

      Women

      0.08-0.60

       Note: 

      Expected range varies from population to population. it is therefore recommended that each laboratory should establish its own normal range. For diagnostic purposes, the GGT results should always be assessed in diagnostic purposes, the bilirubin results should always be assessed in conjuction with the patients medical history clinical examinations and other findings.

      Performance Characteristics  

       Linearity 

      With Autopure Gamma-GT  , the assay is linear up to 1200 IU/I (20.04 ukat/l .Determine samples with higher concentration via the rerun function . On instrument without rerun function, manually dilute samples with higher concentration using  0.9 % NaCL or distilled/deionized water (e.g. 1+4 ). Multiply the result by the appropriate dilution factor (e.g. 5).  

      Interference  

       There is no significant interference in samples containing upto 30 mg/dl of bilirubin and 90 mg/dl of haemoglobin and 10 mg/dl of ascorbic acid.

      Precision

      Reproducibility was determined using quality control sera as shown below  

      Within run

      Between run

      Quality control material

      Means IU/I

      sd IU/I

      CV

      Means IU/I

      sd IU/I

       

      CV

       

      low control serum

      351.363.9371.30

      3.5

      high control serum

      2003.411.7201

      201

      1.6
      1

       Co- Relation Studies

      A comparison of theGGT determination using autopure  Gamma- GT assay (y) and reference GGT kit of Daiichl , japan (x) gave the following  co- realtion (IU/I)

      Linear Regression

      • y=                          -0.636 + 0.998x
      • r=                             0.9835
      • Sy.x=                      9.62

      No of samples measured : 85

      The samples concentrations measured were between 10  and 200 IU/I

      Reference

      • Persijn, J.P., Van der Slik, W. A new method for the determination of -glutamyltransferase. J. Clin. Chem. Clin. Biochemk. 1976;4:421.
      •  Shaw, L.M., Stromme, J.H., London, J.L., et. al. Clin. Chem. Acta 1983; 135 : 315 - 338.
      •  Shaw, L.M. Keeping pace with a popular enzyme GGT. Diagnostic Medicine 1982; May/June: 1-8.
      •  Tietz, N.W. Clinical Guide to Laboratory Tests, 3rd ed. Philadelphia, Pa : WB Saunders Company, 1995 : 286.
      •  In-house test data. Accurex Biomedical Pvt. Ltd., 2002 
      Trade Information
      • EXW ABPL
      • Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
      • 10000 Per Day
      • 2 Days
      • Yes
      • packaging : Empity
      • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
      • All India
      • certification : iso
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      Phone :+918045479256