Glucose 300

Glucose  300
Glucose 300
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 1621 INR
    Product Specifications
    • Other
    • AutoPure Glucose is a single ready to use reagent. With AutoPure Glucose, the assay is linear upto 500 mg/dl (27.75 mmo1/1).
    • Other
    • Biochemistry Reagent
    • other
    • Liquid
    • Biochemistry Reagent
    • 18 Months
    • Reagents
    • Other
    • AC90698
    • Industrial
    • Industrial Lab Chemicals
    • 99%
      Product Description


      • AutoPure Glucose is a reagent kit for direct quantitative determination of glucose in human serum and plasma on automated clinical chemistry analyzers.
      • AutoPure Glucose is a single ready to use reagent.
      • With AutoPure Glucose, the assay is linear upto 500 mg/dl (27.75 mmo1/1).


      • Glucose oxidase, oxidizes glucose to gluconic acid in the presence of atmospheric oxygen.
      • 13- D glucose + 02+ H2O - Gluconic acid + H202
      • In presence of peroxidase, hydrogen peroxide oxidatively couples with 4 - aminoantipyrine and phenol to produce red quinoneimine dye. The intensity of the red colour formed is directly proportional to the concentration of glucose in the specimen and is measured photometrically.
      • H202+ 4 - aminoantipyrine + phenol - Red quinoneimine dye + H20 

      Reagent Storage, Stability & Handling 

      AutoPure Glucose is a single ready to use  reagant.

      Shelf life

      Stable till the expiry date indicated on the label, when stored at 2 -8 C.

      On  Board Reagent Stability

      • 30 days at 2-8 C after opening. Protect the reagent from light and contamination.
      • Over a period of time, the reagent may develop a light pink colour
      • Discard the reagent if the absorbance of the same exceeds 0.300 O.D. against distilled water at 505 nm. (500 - 550 nm).

      Do not freeze the reagent.

      Components & Concentration of Working Solution 

      Components                                                                             Concentration

      • Phosphate buffer; pH 7.2                                              100 mmo1/1
      • Glucose oxidase, recombinant                                        > 180001U/I 
      • Peroxidase                                                                       > 2400 IU/1 
      • 4 - aminoantipyrine                                                            > 0.25 mmo1/1
      • Phenol                                                                                  > 10 mmo1/1
      • Stabilizers, excipients & surface active agents 


      Fully automated system automatically calculates the Glucose concentration of each sample.

      • Results in mmo1/1= Results in mg/di x 0.0555
      • Results in mg/dl = Results in mmo1/1 x 18.02

      Application Sheet 

      Refer to application sheet for details. For additional system applications, contact our local Accurex representative.


      For calibration, it is recommended to use C.f.a.s." or any other suitable calibrator material.

      Calibration frequency

      Re  calibration is recommended

      • Whenever the reagent lot is changed.
      • As per the requirement of quality control procedures. 

      C.f.a.s. is the brand name of Roche 

      Quality Control 

      Each batch of AutoPure Glucose is assayed with atleast six quality control sera - Precinorm, Precipath, Biorad l, Biorad 11, Accutrol Normal and Accutrol Abnormal prior to release.

      To ensure adequate quality control, it is recommended that the laboratory should use a normal and abnormal commercial reference control serum. It should be realized that the use of quality control material checks both the reagent and instrument functions together.

      If the control values fall outside the specified limits, each of the below criteria should be cross  checked and corrected:

      • Proper instrument function  wavelength setting, light source and temperature control.
      • Cleanliness of probes and cuvettes.
      • Bacterial contamination of wash water used by the instrument.
      • Expiry date of the reagent kit.
      Expected Values



      37 C

      Fasting blood glucose

      60 -110 mg/dl

      3.33 - 6.11 mmo1/1

      Postprandial blood glucose

      < 145 mg/dl

      < 8.05 mmo1/1


       Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own normal range. For diagnostic purposes, the glucose results should always be assessed in conjunction with the patient's medical history, clinical examinations and other findings. The whole blood glucose value is generally 10 - 15% lower than the serum or plasma glucose value due to the cell stroma.

      Performance Characteristics 


      With AutoPure Glucose, the assay is linear upto 500 mg/dl (27.75 mmo1/1). Determine samples with higher concentrations via the rerun function. On instruments without rerun function, manually dilute samples with higher concentrations using 0.9% NaCI or distilled/deionized water (e.g.1 + 2). Multiply the result by the appropriate dilution factor (e.g. 3). 


      There is no significant interference in samples containing upto 20 mg/dl of bilirubin and 750 mg/dl of haemoglobin.


       Reproducibility was determined using quality control sera as shown below:

      Within run

      Between run

      Quality Control Material

      Mean mg/dL

      SD mg/dL


      Mean mg/dL

      SD mg/dL


      Low Contro







      High Contol







       Co  Relation Studies

      A comparison of the glucose determination using AutoPure Glucose and Infinite Glucose reagent gave the following co-relation (mg/di):

      Linear Regression

      • y = -1.863 + 1.042x
      • r = 0.9976
      • Sy.x = 2.11

      No. of samples measured : 89

      The sample concentrations measured were between 52 and 160 mg/dl


      • Trinder P. Annals. Clin. Biochem 6,24 (1969).
      • Young D.S. etal., Clinical Chemistry, 21,1d (1975).
      • Bergmayer H.V., Methods of Enzymatic Analysis, A.P.,N.Y. (1974).Page 1196.
      • In-house test data. Accurex Biomedical Pvt. Ltd., 2014
      Trade Information
      • EXW ABPL
      • Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
      • 10000 Per Day
      • 2 Days
      • Yes
      • packaging : Empity
      • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
      • All India
      • certification : iso
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      Contact Us

      212, Udyog Mandir No. 17/C, Bhagoji Keer Marg, Mahim, Mumbai, Maharashtra, 400016, India
      Phone :+918045479256