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Glucose Hexokinase 200

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Glucose Hexokinase 200
Glucose Hexokinase 200
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 3987 INR
    Product Specifications
    • Powder
    • Biochemistry Reagent
    • Other
    • Other
    • Biochemistry Reagent
    • 18 Months
    • other
    • Autopure Glucose Hexokinase is a ready to use two liquid reagent system With Autopure Glucose Hexokinase the assay is linear upto 600 mgdL
    • Reagents
    • Other
    • ACC9064
    • Industrial
    • Industrial Lab Chemicals
    • 99%
      Product Description

      Introduction

      • Autopure Glucose Hexokinase is a reagent kit for quantitative determination of Glucose concentration in human serum or plasma based on Hexokinase method
      • Autopure Glucose Hexokinase is a ready to use two liquid reagent system
      • With Autopure Glucose Hexokinase the assay is linear upto 600 mgdL

      Clinical Significance

      Estimation of glucose concentration is important in the diagnosis and management of hyperglycaemia and hypoglycaemia Condition of Hyperglycaemia may occur as a result of diabetes mellitus patients intravenously receiving glucose containing fluids during severe stress and cerebrovascular accidents Hypoglycaemia may be the result of an insulinoma insulin administration inborn errors of carbohydrate metabolism or fasting

      Reagent Storage Stability  Handling

      Autopure Glucose  Hexokinase is a ready to use two liquid reagent system

      Shelf life

      Unopened reagents are stable till the expiry date indicated on the label when stored at 2   8 C and protected from light

      OnBoard Reagent Stability

      Once opened the reagents are stable up to 60 days at 2 8 C  if protected from light and contamination Open bottle stability may vary from analyzer to analyzer Do not freeze the reagent  

      Components & Concentration of Working Solution

       Components                                                                                           Concentration

      • Hexokinase                                                                                       >1500 U/L 
      • G6PHD                                                                                              > 2000 U/L 
      • ATP                                                                                                       >1 mmol/L 
      • Coenzymes, stabilizers & excipients

      Specimen Collection & Preservation

      Collect sample using standard sampling tube. Serum or plasma (heparin or fluoride) is the specimen of choice. Serum or plasma should be separated from the cell within 30 minutes  as the rate of glycolysis is approximately 7mg/dL per hour at room temperature. Sodium fluoride is preferred as anticoagulant due to its antiglycolytic activity Glucose in samples is stable for 1 day if stored at 2 -8C

      Calculations

      Fully automated system automatically calculates the Glucose concentration of each sample.

      Calibration

      For calibration  it is recommended to use c.f.a.s. or Accucal or any other suitable calibrator material.  

      Calibration frequency

      The frequency of calibration is dependent on the analyzer. Re calibration is recommended

      •  Whenever the reagent lot is changed.
      • As per the requirement of quality control procedures.  

      Quality Control

      Each batch of Autopure Glucose (Hexokinase) is assayed with atleast six control sera   Precinorm, Precipath, Biorad I Biorad II, Accutrol normal and Accutrol Abnormal prior to release  .

      To ensure adequate quality control  it is recommended that the laboratory should use a normal and an abnormal commercial reference control material. It should be realized that the use of quality control material checks both reagent and instrument functions together. 

      If the control values fall outside the specified limits, each of the below criteria should be cross-checked and corrected:

      •  Proper instrument function  wavelength setting  light source and temperature control
      •  Cleanliness of probes and cuvettes.
      •  Bacterial contamination of wash water used by the instrument
      •  Expiry date of the reagent kit 

      Expected Values

      Fasting

      60 - 110 mg/dL (3.33 - 6.11 mmol/L)

      Postprandial

      < 145 mg/dL (< 8.05 mmol/L)

       Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own reference range. For diagnostic purposes, Glucose results should always be assessed in conjunction with the patients medical history clinical examination and other findings.

      Performance Characteristics

      Linearity

      With Autopure Glucose (Hexokinase) the assay is linear upto 600 mg/dL. Determine samples with higher concentration via the rerun function. On instruments without rerun function, manually dilute samples with higher concentration using normal saline (0.9% NaCl) or distilled/deionized water and repeat the assay. The result obtained should be then multiplied with dilution factor to obtain correct Glucose concentration

      Precision

      Reproducibility was determined using quality control sera as shown below: 

      n = 20

      Within run

      Between run

      Quality Control Material

      Mean mg/dL

      SD mg/dL

      CV

      Mean mg/dL

      SD mg/dL

      CV

      Low Contro

      93

      0.76

      0.82

      96.5

      1.83

      1.9

      High Contol

      235.5

      1.81

      0.77

      239

      2.89

      1.21

       Correlation Studies

      A comparison of Glucose determination using Autopure Glucose Hexokinase and reference competitors product gave the following correlationmgdL

      Linear Regression

      • y  10123x  14318
      • R2  09983

      References 

      • Tietz N W Ed Fundamentals of Clinical Chemistry W B Saunders Co Philadelphia 
      • CLSI Method Evaluation Protocols Clinical  Laboratory Standards Institute Wayne PA
      • Young DS Effects of Drugs on Clinical Laboratory Tests 3rd ed  AACC Press Washington 1990
      • Inhouse test data Accurex Biomedical Pvt Ltd 2018  
      Trade Information
      • EXW ABPL
      • Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
      • 10000 Per Day
      • 2 Days
      • packaging : Empity
      • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
      • All India
      • certification : iso
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        212, Udyog Mandir No. 17/C, Bhagoji Keer Marg, Mahim, Mumbai, Maharashtra, 400016, India
        Phone :+918045479256