GOT 30

• Reaction type: UV - Kinetic
• Reaction direction: Down,
• Wavelength: 340 nm.
• Flowcell temperature: 37 degree C
• Zero setting with: Distilled water
• Delay time: 60 seconds
• No. of readings :4
• Interval: 30 seconds
• Blank absorbance limit:  0.900 Abs.
• Sample volume: 0.1 ml (100 p1)
• Reagent volume: 1.0 ml
• Factor: 1749

Mix and aspirate. After the initial delay of 60 seconds, record the absorbance of the test at an interval of 30 seconds for the next 90 seconds at 340nm. Determine the mean change in absorbance per minute and calculate test results.

EXPECTED VALUES

Up to 46 IU/1 at 37 degree C

The expected value should be used as a guide only. It is recommended that each laboratory should establish its own normal range.

PROCEDURE LIMITATIONS

• Hemolysis must be avoided because erythrocytes contain approximately ten times the normal concentration of GOT (AST) found in serum.
• If the GOT activity exceeds 400 IU/I, dilute the specimen and carry out the assay. In such cases the results obtained should be multiplied by dilution factor to obtain correct GOT activity.
• The working solution is considered unsatisfactory and should not be used if the absorbance is less than 0.900 at 340 nm. against distilled water.

QUALITY CONTROL

To ensure adequate quality control, it is recommended that each batch should include a normal and abnormal commercial reference control serum. It should be realised that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature.