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GOT 30

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GOT 30
GOT 30
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 560 INR
    Product Specifications
    • Other
    • other
    • In - vitro Diagnostics
    • Liquid
    • 18 Months
    • Auto Zyme GOT is a single reagent system, using one step procedure. Auto Zyme GOT is a High Stability Reagent. Auto Zyme GOT is linear up to 400 IU/1.
    • Other
    • In - vitro Diagnostics
    • Reagents
    • Other
    • ACC66363
    • Industrial
    • Industrial Lab Chemicals
    • 95
      Product Description
      INTRODUCTION

      • Auto Zyme GOT is a reagent set for determination of GOT (AST) activity in serum or plasma. The reaction is based on UV-Kinetic method recommended by the International Federation of Clinical Chemistry (IFCC).
      • Auto Zyme GOT is a single reagent system, using one step procedure.
      • Auto Zyme GOT is a High Stability Reagent.
      • Auto Zyme GOT is linear up to 400 IU/1.
      • Auto Zyme GOT can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request.
      • GOT activity can be determined in 21/2 minutes.
      PRINCIPLE

      The conversion of NADH to WAD+ is proportional to the concentration of GOT in serum and is measured at 340 nm. as rate of decrease in absorbance.

      Abbreviations
      • GOT : Glutamate Oxaloacetate Transaminase
      • AST:  Aspartate Transaminase
      PREPARATION OF WORKING SOLUTION

      Reconstitute reagents as per instruction on individual bottle label to prepare working solution. Mix by gentle swirling or inversion. Do not shake vigorously.

      REAGENT STORAGE & STABILITY
      • The reagent kit should be stored at 2 - 8 degree C and is stable till the expiry date indicated on the label.
      • The GOT working solution is stable for 60 days at 2-8 degree C. Do not freeze the working solution.
      COMPONENTS & CONCENTRATION OF WORKING SOLUTION

      Component

      Concentration

      Tris buffer

      80 mM (pH 7.7)

      L-Aspartate

      200 mM

      NADH

      0.15 mM

      LDH

      > 200 IU/1

      GC-Ketoglutarate

      12 mM

      MDH

      3001U/I


      SPECIMEN COLLECTION  & PRESERVATION

      Although serum is preferred, plasma can also be used. Anticoagulants such as heparin and EDTA can be used. Blood samples may be collected any time, although morning samples are preferred.
      Samples with any visible haemolysis are not acceptable. Samples are stable for a week at 2-8 degree C and for one month at -10 degree C. Samples should be brought to room temperature prior to use.

      PROCEDURE
      • Reaction type: UV - Kinetic
      • Reaction direction: Down,
      • Wavelength: 340 nm.
      • Flowcell temperature: 37 degree C
      • Zero setting with: Distilled water
      • Delay time: 60 seconds
      • No. of readings :4
      • Interval: 30 seconds
      • Blank absorbance limit:  0.900 Abs.
      • Sample volume: 0.1 ml (100 p1)
      • Reagent volume: 1.0 ml
      • Factor: 1749
      Manual assay procedure

      Prewarm at 37 degree C the required amount of working solution before use.

      1.0 ml procedure

       

      • Serum / Plasma: 0.1 ml
      • Working Solution :  1.0 ml

       

      Mix and aspirate. After the initial delay of 60 seconds, record the absorbance of the test at an interval of 30 seconds for the next 90 seconds at 340nm. Determine the mean change in absorbance per minute and calculate test results.

      EXPECTED VALUES

      Up to 46 IU/1 at 37 degree C

      The expected value should be used as a guide only. It is recommended that each laboratory should establish its own normal range.

      PROCEDURE LIMITATIONS

       

      • Hemolysis must be avoided because erythrocytes contain approximately ten times the normal concentration of GOT (AST) found in serum.
      • If the GOT activity exceeds 400 IU/I, dilute the specimen and carry out the assay. In such cases the results obtained should be multiplied by dilution factor to obtain correct GOT activity.
      • The working solution is considered unsatisfactory and should not be used if the absorbance is less than 0.900 at 340 nm. against distilled water.

       

      QUALITY CONTROL

      To ensure adequate quality control, it is recommended that each batch should include a normal and abnormal commercial reference control serum. It should be realised that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature 

      Trade Information
      • EXW ABPL
      • Paypal, Letter of Credit at Sight (Sight L/C), Letter of Credit (L/C), Cheque, Cash in Advance (CID), Cash Against Delivery (CAD)
      • 10000 Per Day
      • 2 Days
      • Yes
      • packaging : Empity
      • Africa, Middle East, Western Europe, Eastern Europe, South America, North America, Central America, Australia, Asia
      • All India
      • certification : iso
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      Phone :+918045479256