0
home

GOT (AST) 220

home
GOT (AST) 220
GOT (AST) 220
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 5349 INR
    Product Specifications
    • Other
    • AutoPure AST GOT is a ready to use two liquid reagent system With AutoPure AST GOT the assay is linear upto 800 IUI 1336 ukatI
    • Liquid
    • Other
    • 18 Months
    • Biochemistry Reagent
    • other
    • Biochemistry Reagent
    • Reagents
    • Other
    • ACC90600
    • Industrial
    • Industrial Lab Chemicals
    • 99%
      Product Description

       Introduction

      •  AutoPure AST GOT is a reagent kit for direct quantitative determination of aspartate transaminase AST in human serum and plasma on automated
      • clinical chemistry analyzers
      •  AutoPure AST GOT is a ready to use two liquid reagent system
      • With AutoPure AST GOT the assay is linear upto 800 IUI 1336 ukatI
      Principle
      • Ketoglutarate reacts with L aspartate in the presence of AST GOT to form oxaloacetate and L glutamate
      • Ketoglutarate  Laspartate oxaloacetate  L glutamate
      • The increase in oxaloacetate is determined in an indicator reaction catalyzed by malate dehydrogenas oxaloacetate  NADH  H Lmalate  NAD
      • The conversion of NADH to NAD  is proportional to the AST GOT activity in the specimen and is determined kinetically 

      Reagent Storage Stability  Handling

      AutoPure AST GOT is a ready to use two liquid reagent system

      Shelf  Life 

      Stable till the expiry date indicated on the label when stored at 20  80C

      On - Board Reagent Stability

      • R1  30 days at 20 - 80 C after opening. Protect the reagent from light andcontamination.
      • R2  30 days at 20 - 80 C after opening. Protect the reagent from light and contamination.

      Do not freeze the reagent 

      Specimen Collection & Preservation

      Collect sample using standard sampling tube. Although serum is preferred, heparinized plasma or EDTA  plasma can also be used. Specimen with any visible haemolysis is not acceptable, since erythrocytes contain approximately ten times the normal concentration of AST (GOT) found in serum. AST (GOT) in serum  plasma is stable for 7 days at 20  - 80C and for 30 days at -100C. Centrifuge samples containing precipitate before performing assay

      Calculations

      • Fully automated systems automatically calculate the AST (GOT) activity of each sample.
      • Results in ukat/l = Results in IU/I x 0.0167

      Application Sheets

      Application sheets for the popular fully automated analyzers are provided along with the kit. For additional system applications, contact our local Accurex representative.

      Calibration

      Determine the factor using a calibrator. For calibration, it is recommended to use C.f.a.s.or any other suitable calibrator material 

      Calibration frequency :

      Re calibration is recommended

      •  Whenever the reagent lot is changed
      •  As per the requirements of quality control procedures 
      Quality Control

      Each batch of AutoPure AST (GOT) is assayed with at least six quality control sera  Precinorm, Precipath  Biorad l  Biorad II, Sero Low and Sero High prior to release

      To ensure adequate quality control, it is recommended that the laboratory should use a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both reagent and instrument functions together. The value of these controls should fall within the specified limits. If control values fall outside specified limits, each of the below criteria should be cross-checked and corrected :

      •  Proper instrument function  wavelength setting, light source and temperature control
      • Cleanliness of probes & cuvettes
      •  Bacterial contamination of wash water used by the instrument
      •  Expiry date of the reagent kit.
      Expected Values

      Serum/Plasma

       

       

      25 C

      30 C

      37 C

      IU/I

      Men

      Upto 18

      Upto 25

      Upto 37

       

      Women

      Upto 15

      Upto 21

      Upto 31

      ukat/l

      Men

      Upto 0.30

      Upto 0.42

      Upto 0.62

       

      Women

      Upto 0.25

      Upto 0.35

      Upto 0.52

      The following factors are used for conversion :

      • From 370 C to 300 C : 0.67
      • From 370 C to 250 C : 0.49 

       Note :

      Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own normal range. For diagnostic purposes, the AST (GOT) results should always be assessed in conjunction with the patient s medical history, clinical examinations and other findings.

      Performance Characteristics

      Linearity

      With AutoPure AST (GOT), the assay is linear upto 800 IU/I (13.36 ukat/l). Determine samples with higher concentrations via the rerun function. On instruments without rerun function, manually dilute samples with higher concentrations using 0.9  NaCl or distilled / deionized water (e.g. 1 + 2). Multiply the result by the appropriate dilution factor (e.g. 3).

      Interference

      There is no significant interference in samples containing upto 60 mg/dl of bilirubin. Haemolysis interferes due to AST activity from erythrocytes.

      Precision

      Reproducibility was determined using quality control sera as shown below: 


      Within run

      Between run

       

      Quality control material

      Means 1U/1

      sd 1U/1

      CV

      Means 1U/1

      sd 1U/1

      CV

      low control serum

      37

      1.29

      3.5

      37

      1.27

      3.5

      high control serum

      162

      2.21

      1.4

      158

      2.83

      1.8

       Co-Relation Studies

      A comparison of AST (GOT) determination using AutoPure AST (GOT) and AutoZyme SGOT reagent gave the following co-relation (IU/I):

      Linear Regression

      • y = -0.114 + 1.011x
      • r = 0.986
      • Sy.x = 1.88

      No. of samples measured : 58

      The sample concentrations measured were between 15 and 65 IU/I

      References
      •  Tietz NW ed Clinical Guide to Laboratory Tests 3rd ed Philadelphia Pa  WB Saunders 1995  76  77
      •  Bergmeyer HU Horder M Rej R Approved recommendation 1985 on IFCC methods for the measurement of catalytical concentration of enzymes Part 3 IFCC method for L  aspartate aminotransferase J Clin Chem Clin Biochem 1986 24  497  510
      •  Fischbach F Zawta B Age  depending Reference Limits of Several Enzymes in plasma at Different Measuring Temperatures Klin Lab 1992 38555  561
      • Penttila IM et al Scand J Clin Lab Invest 35 275  1975
      • Hafkenshelid JCM et al J Clin Chem Clin Biochem 17 219 1979
      •  Inhouse test data Accurex Biomedical Pvt Ltd 2002 
      Trade Information
      • EXW ABPL
      • Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
      • 10000 Per Day
      • 2 Days
      • Yes
      • packaging : Empity
      • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
      • All India
      • certification : iso
      Related Products
      BUN 250
















      Send Inquiry
      Amylase 60 - Infinite
















      Send Inquiry
      CK NAC 30
















      Send Inquiry
      Turbilatex ASO Infinite
















      Send Inquiry
      AutoPure Homocysteine 5 Level Calibrator















      Send Inquiry
      Infinite Cholestrol 600





      Send Inquiry
      right arrow
      left arrow
      Contact Us

      212, Udyog Mandir No. 17/C, Bhagoji Keer Marg, Mahim, Mumbai, Maharashtra, 400016, India
      Phone :+918045479256