GPT (ALT) 100

a.-ketoglutarate reacts with L-alanine in presence of GPT (ALT) to form pyruvate and L-glutamate. The increase in pyruvate is determined in an indicator reaction catalyzed by lactate dehydrogenase. The conversion of NADH to NAD at 340 nm. is proportional to the activity of GPT (ALT) in serum/plasma and is determined kinetically as rate of decrease in absorbance.

Abbreviations

• ALT :  Alanine transaminase
• GPT : Glutamate pyruvate transaminase

PREPARATION OF WORKING SOLUTION

Prepare working solution by mixing Reagent R, and Reagent R, in the ratio 4: 1 as per requirement. 

REAGENT STORAGE STABILITY

• The reagent kit should be stored at 2 - 8 degree C and is stable till the expiry date indicated on the label. .
• R, and R, reagents are stable till expiry at 2 - 8 degree C.
• The working solution (4 R, + 1 R,) is stable for 30 days at 2 - 8 degree C. 

PROCEDURE LIMITATIONS

• If the GPT (ALT) activity exceeds 600 IU/I, dilute the specimen with normal saline and repeat the assay. The result obtained should then be multiplied with the dilution factor to obtain correct GPT (ALT) activity.
• The working solution is considered unsatisfactory and should not be used if the absorbance is less than 0.900 at 340 nm. against distilled water.

QUALITY CONTROL

To ensure adequate quality control, it is recommended that each batch should include normal and abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.