GPT (AST) 220

GPT (AST) 220
GPT (AST) 220
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 5349 INR
    Product Specifications
    • Industrial
    • Industrial Lab Chemicals
    • 99%
    Product Description


    •  AutoPure AST GOT is a reagent kit for direct quantitative determination of aspartate transaminase AST in human serum and plasma on automated
    • clinical chemistry analyzers
    •  AutoPure AST GOT is a ready to use two liquid reagent system
    • With AutoPure AST GOT the assay is linear upto 800 IUI 1336 ukatI
     ketoglutarate reacts with L aspartate in the presence of AST GOT to form oxaloacetate and L glutamate

    ketoglutarate  Laspartate oxaloacetate  L glutamate

    The increase in oxaloacetate is determined in an indicator reaction catalyzed by malate dehydrogenas

    oxaloacetate  NADH  H Lmalate  NAD

    The conversion of NADH to NAD  is proportional to the AST GOT activity in the specimen and is determined kinetically 

    Reagent Storage Stability  Handling

    AutoPure AST GOT is a ready to use two liquid reagent system

    Shelf  Life 

    Stable till the expiry date indicated on the label when stored at 20  80C

    On - Board Reagent Stability

    R1  30 days at 20 - 80 C after opening. Protect the reagent from light andcontamination.

    R2  30 days at 20 - 80 C after opening. Protect the reagent from light and contamination.

    Do not freeze the reagent 

    Components & Concentration of Working Solution

     Components                                                                                   Concentration 


    •   Tris buffer  pH 7.8                                                                            100 mmol/l
    • L aspartate                                                                                          > 200 mmol/l
    •  Malate dehydrogenase                                                                         > 400 IU/I
    •  Lactate dehydrogenase                                                                         > 1500 IU/I
    •  Stabilizers excipients & surface active agents.


    • Tris buffer; pH 9.5                                                                             20 mmol/l
    • a ketoglutarate                                                                                   > 10 mmol/l
    •  NADH                                                                                               > 0.18 mmol/l
    •  Stabilizers, excipients & surface active agent 
    Specimen Collection & Preservation

    Collect sample using standard sampling tube. Although serum is preferred, heparinized plasma or EDTA  plasma can also be used. Specimen with any visible haemolysis is not acceptable, since erythrocytes contain approximately ten times the normal concentration of AST (GOT) found in serum. AST (GOT) in serum  plasma is stable for 7 days at 20  - 80C and for 30 days at -100C. Centrifuge samples containing precipitate before performing assay


    Fully automated systems automatically calculate the AST (GOT) activity of each sample.

    Results in ukat/l = Results in IU/I x 0.0167

    Application Sheets

    Application sheets for the popular fully automated analyzers are provided along with the kit. For additional system applications, contact our local Accurex representative.


    Determine the factor using a calibrator. For calibration, it is recommended to use C.f.a.s.or any other suitable calibrator material 

    Calibration frequency :

    Re calibration is recommended

    •  Whenever the reagent lot is changed
    •  As per the requirements of quality control procedures 
    Quality Control

    Each batch of AutoPure AST (GOT) is assayed with at least six quality control sera  Precinorm, Precipath  Biorad l  Biorad II, Sero Low and Sero High prior to release

    To ensure adequate quality control, it is recommended that the laboratory should use a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both reagent and instrument functions together. The value of these controls should fall within the specified limits. If control values fall outside specified limits, each of the below criteria should be cross-checked and corrected :

    •  Proper instrument function  wavelength setting, light source and temperature control
    • Cleanliness of probes & cuvettes
    •  Bacterial contamination of wash water used by the instrument
    •  Expiry date of the reagent kit.
    Expected Values




    25 C

    30 C

    37 C



    Upto 18

    Upto 25

    Upto 37



    Upto 15

    Upto 21

    Upto 31



    Upto 0.30

    Upto 0.42

    Upto 0.62



    Upto 0.25

    Upto 0.35

    Upto 0.52

     The following factors are used for conversion :

    From 370 C to 300 C : 0.67

    From 370 C to 250 C : 0.49 

     Note :

    Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own normal range. For diagnostic purposes, the AST (GOT) results should always be assessed in conjunction with the patient s medical history, clinical examinations and other findings.

    Performance Characteristics


    With AutoPure AST (GOT), the assay is linear upto 800 IU/I (13.36 ukat/l). Determine samples with higher concentrations via the rerun function. On instruments without rerun function, manually dilute samples with higher concentrations using 0.9  NaCl or distilled / deionized water (e.g. 1 + 2). Multiply the result by the appropriate dilution factor (e.g. 3).


    There is no significant interference in samples containing upto 60 mg/dl of bilirubin. Haemolysis interferes due to AST activity from erythrocytes.


    Reproducibility was determined using quality control sera as shown below: 

    Within run

    Between run


    Quality control material

    Means 1U/1

    sd 1U/1


    Means 1U/1

    sd 1U/1


    low control serum







    high control serum







     Co-Relation Studies

    A comparison of AST (GOT) determination using AutoPure AST (GOT) and AutoZyme SGOT reagent gave the following co-relation (IU/I):

    Linear Regression

    y = -0.114 + 1.011x

    r = 0.986

    Sy.x = 1.88

    No. of samples measured : 58

    The sample concentrations measured were between 15 and 65 IU/I

    •  Tietz NW ed Clinical Guide to Laboratory Tests 3rd ed Philadelphia Pa  WB Saunders 1995  76  77
    •  Bergmeyer HU Horder M Rej R Approved recommendation 1985 on IFCC methods for the measurement of catalytical concentration of enzymes Part 3 IFCC method for L  aspartate aminotransferase J Clin Chem Clin Biochem 1986 24  497  510
    •  Fischbach F Zawta B Age  depending Reference Limits of Several Enzymes in plasma at Different Measuring Temperatures Klin Lab 1992 38555  561
    • Penttila IM et al Scand J Clin Lab Invest 35 275  1975
    • Hafkenshelid JCM et al J Clin Chem Clin Biochem 17 219 1979
    •  Inhouse test data Accurex Biomedical Pvt Ltd 2002 
    Trade Information
    • EXW ABPL
    • Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
    • 10000 Per Day
    • 2 Days
    • Yes
    • packaging : Empity
    • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
    • All India
    • certification : iso
    Related Products
    Turbilatex CRP Infinite

    Send Inquiry
    Microprotein 100

    Send Inquiry
    Turbilatex RF Infinite

    Send Inquiry
    LDL Cholesterol Direct 40

    Send Inquiry
    Cystatine C control low And High

    Send Inquiry
    BUN 200

    Send Inquiry
    right arrow
    left arrow
    Contact Us

    212, Udyog Mandir No. 17/C, Bhagoji Keer Marg, Mahim, Mumbai, Maharashtra, 400016, India
    Phone :+918045479256