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HDL Cholestrol 100

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HDL Cholestrol 100
HDL Cholestrol 100
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 1018 INR
    Product Specifications
    • Industrial
    • Industrial Lab Chemicals
      Product Description
      INTRODUCTION
      • Auto Zyme HDL-Cholesterol Precipitating Reagent is for use in conjunction with Auto Zyme Cholesterol Reagent - for enzymatic determination of HDL-Cholesterol in serum or plasma.
      • Compared to the conventional Ultra-centrifugation method, the precipitation method is simple and time saving; particularly when combined with single-step enzymatic Auto Zyme Cholesterol reagent.
      PRINCIPLE

      Phosphotungstate/Mg2+ precipitates chylomicrons, LDL and VLDL fractions. High Density Lipoprotein (HDL) fraction remains un effected in supernatant. Cholesterol content of HDL fraction is assayed using Auto Zyme Cholesterol.

      PREPARATION OF WORKING SOLUTION

      HDL - Cholesterol Precipitating Reagent is ready-to-use as supplied

      COMPONENTS & CONCENTRATION OF PRECIPITATING REAGENT

      Component

      Concentration

      Phosphotungstic acid

      2.4 mmo1/1

      Magnesium Chloride

      40 mmo1/1

      SPECIMEN COLLECTION  & PRESERVATION

      Blood should be collected in a clean dry container. Fasting blood is preferred for HDL-Cholesterol assay.

      Plasma should be separated immediately from the cells. For plasma separation any of the following anticoagulants may be used:

      • EDTA 10 mg/ml blood
      • HEPARIN 200 Um blood

      HDL-Cholesterol value is stable in serum for 24 hours at 2-8 degree C and 30 days 

      PROCEDURE

      HDL separation

      Prewarm at room temperature (25 - 30 degree C) the required amount of Precipitating Reagent and Auto Zyme Cholesterol working solution before use.

      Perform the assay as given below. 

      Pipette as follows : 

      • Serum/plasma: 0.5 ml
      • HDL-Precipitating reagent: 0.5 ml

      Mix thoroughly and centrifuge at 4000 r.p.m. for 10 minutes in a common laboratory centrifuge (1800 x g) to obtain a clear supernatant.

      HDL-Cholesterol determination

      • Reaction type: End-Point
      • Reaction time: 10 mins. at 37 degree C130 mins. at R.T. (25-30 degree C)
      • Wavelength: 510 nm. (505-530 nm.)
      • Zero setting with : Reagent Blank
      • Blank absorbance limit:  0.100 Abs.
      • Sample volume: 0.05 m1(50 gl)
      • Reagent volume : 1.0 ml
      • Standard concentration: 50 mg%
      • Linearity: 400 mg%
      Manual assay procedure

      Assay the supernatant for HDL-Cholesterol within 2 hours after centrifugation using working solution of Auto Zyme Cholesterol Reagent.

      Incubation

      Incubate the assay mixture for 10 minutes at 37 degree C or 30 minutes at room temperature (25-30 degree C). After completion of the incubation, measure the absorbance of assay mixture against blank at 510 nm. Final colour is stable for two hours, if not exposed to direct light.

      EXPECTED VALUES

      30 - 70 mg%

      CLINICAL SIGNIFICANCE 

      OF HDL - CHOLESTEROL

      HDL - Cholesterol values below 30 mg% indicate towards the risk factor. The clinical risk factor is better represented by the ratio of Total Cholesterol to HDL Cholesterol in case of heart ailments.

      Risk Factor

      Total Chol. / HDL Chol

       

      Female

      Male

      Average x 0.5

      3.3

      3.4

      Average x 1.0

      4.5

      5.0

      Average x 2.0

      7.0

      9.6

      Average x 3.0

      11.0

      24.0

       Clinical interpretation

      Criteria for the treatment of lipid disorders :

       

       

      Treatment required

       

       

      No

      Depending on the
      HDL and / or LDL
      Cholesterol level

      Yes

      Total Cholesterol' (mg/100 ml )

      < 200

      200 - 300

       

      Triglycerides (mg/100 ml )

      < 200

      > 200

       

       

       

      Treatment required

       

       

      No

       

      Yes

      HDL Cholesterol' (mg/100 ml )

      > 35

       

      < 35

      LDL Cholesterol 2 (mg/100 ml )

      < 150

       

      > 190

      QUALITY CONTROL

      To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might effect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.

      Trade Information
      • EXW ABPL
      • Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
      • 10000 Per Day
      • 2 Days
      • Yes
      • packaging : Empity
      • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
      • certification : iso
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        212, Udyog Mandir No. 17/C, Bhagoji Keer Marg, Mahim, Mumbai, Maharashtra, 400016, India
        Phone :+918045479256