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Lipase 30

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Lipase 30
Lipase 30
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 8509 INR
    Product Specifications
    • other
    • AutoPure Lipase is a ready to use two liquid reagent system With AutoPure Lipase the assay is linear upto 300 IU1 501 p katI
    • Biochemistry Reagent
    • Biochemistry Reagent
    • Liquid
    • Other
    • 18 Months
    • Other
    • Reagents
    • Other
    • ACC9057
    • Industrial
    • Industrial Lab Chemicals
    • 95%
      Product Description

       Introduction

      • AutoPure Lipase is reagent kit for direct quantitative determination of Lipase in human serum and plasma on automated clinical chemistry analyzers
      • AutoPure Lipase is a ready to use two liquid reagent system
      • With AutoPure Lipase the assay is linear upto 300 IU1 501 p katI
      Principle

      The chromogenic Lipase substrate 12  o  dilauryl  rac  glycero  3 glutaric acid6methylresorufin ester is cleaved by the catalytic action of lipase to form 12  o  dilauryl  rac  glycerol and an unstable intermediate glutaric acid  6  methylresorufin ester This decomposes spontaneously in alkaline medium to form glutaric acid and methylresorufin
      • 12  o  dilauryl  rac  glycero  3  glutaric acid  6  methylresorufin easter
      • 1 2  o  dilauryl  rac  glycerol  Glutaric acid   6  methylresorufin ester
       The Lipase activity in specimen is directly proportional to the rate of formation of methylresorufin in the reaction and can be determined kinetically

       Reagent Storage Stability  Handling 

       AutoPure Lipase is a ready to use two liquid reagent 

      Shelf life

      Stable till the expiry date indicated on the label when stored at 28C 

      On   Board Reagent Stability

      • R1 28 days at 2 8C after opening
      • R2  28 days at 2 8C after opening
      • Protect the reagent from light and contamination
      • Do not freeze the reagent 

      Components & Concentration of Working Solution 

      Components                                                                         Concentration

      • BICIN buffer, pH 8.0                                                                50 mmo1/1
      • Colipase                                                                                       > 1 mg/I
      • Sodium deoxycholate                                                                  1.6 mmo1/1                                                            
      • Calcium chloride                                                                        10 mmo1/1
      R2

       Components                                                                                          Concentration

      • Tartatate buffer, pH 4.0                                                                               50 mmo1/1
      • Taurodeoxycholate                                                                                     8.8 mmo1/1
      • DGGMR*                                                                                                  0.027 mmo1/1
      • Stabilizers, excipients & surface active agents.

       Specimen Collection  Preservation

      Collect sample using standard sampling tube Serum heparinized plasma with Lithium or Sodium can be used Lipase activity in serumplasma is stable for 5 days at 28C and 24 hours at 2025C Centrifuge samples containing precipitate before performing the assay  

       Application Sheet 

       Refer to the application sheet for details For additional system applications contact our local Accurex representative

       Calibration

      For calibration it is recommended to use Cfas or any other suitable calibrator material

       Calibration frequency

      Re  calibration is recommended

      • Whenever the reagent lot is changed
      • As per the requirement of quality control   

       Quality Control

      Each batch of AutoPure Lipase is assayed with atleast six quality control sera  Precinorm Precipath Biorad 1 Biorad 11 Accutrol Normal and Accutrol Abnormal prior to release

       To ensure adequate quality control it is recommended that the laboratory should use a normal and abnormal commercial reference control serum It should be realized that the use of quality control material checks both the reagent and instrument functions together

      If the control values fall outside the specified limits each of the below criteria should be cross  checked and corrected

      • Proper instrument function  wavelength setting light source and temperature control
      • Cleanliness of probes and cuvettes
      • Bacterial contamination of wash water used by the instrument
      • Expiry date of the reagent kit

       Expected Values

      SerumPlasma

      Adults  13  60 IU1 02171002 pkatl at 37 C

      Note

      Expected range varies from population to population It is therefore recommended that each laboratory should establish its own range For diagnostic purposes the Lipase results should always be assessed in conjunction with the patients medical history clinical examinations and other findings 

       Performance Characteristics

      Linearity 

      With AutoPure Lipase the assay is linear upto 300 IU1 501 pkatl Determine samples with higher concentrations via the rerun function On instruments without rerun function manually dilute samples with higher concentrations using 09 NaCI or distilleddeionized water eg 1  4 Multiply the result by the appropriate dilution factor eg 5 

      Interference

       There is no significant interference in samples containing upto 60 mgdl of bilirubin and 500 mgdl of haemoglobin 

      Precision

      Reproducibility was determined using quality control sera as shown below

      Within run

      Between run

      Quality Control Material

      Means 1U/1

      SD 1U/1

      CV

      Means 1U/1

      SD 1U/1

      CV

      Low Control Serum

      13.3

      1.52

      1.1

      140

      1.85

      1.4

      High Control Serum

      456

      2.53

      0.6

      475

      2.32

      0.9

       Co  Relation Studies

      Acomparison of the Lipase determination using AutoPure Lipase and Infinite Lipase reagent gave the following corelation IU1

      Linear Regression

      • y  0304x  656
      • r  0986
      • Syx 174

      No of samples measured  72

      The sample concentrations measured were between 5 and 661 IU1 

      References

      • Tietz N W etal Lioase in Serum  the elusive enzyme An overview Clin Chem1993 39746756
      • Tietz NW etal Clinical guide to Laboratory Tests WB Saunders Philadelphia p 865 1995
      • Leybold A Jung W Imprtance of Colipase for the measurement of serum lipase activity Adv Clin EnzymoL 1986 46067
      • Pentaghini M etal Diagnostic value of four assays for lipase determination in serum A comparative reevaluation Clin Biochem 1991 24497503
      • Steinberg W M Goldstein SS Davies ND etal Diagnostic assays in acute pancreatitis Review Ann Intern Med 1985 102576580 
      Trade Information
      • EXW ABPL
      • Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
      • 10000 Per Day
      • 2 Days
      • Yes
      • packaging : Empity
      • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
      • All India
      • certification : iso
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      212, Udyog Mandir No. 17/C, Bhagoji Keer Marg, Mahim, Mumbai, Maharashtra, 400016, India
      Phone :+918045479256