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Magnesium 80
Magnesium 80
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  • Kit/Kits
  • 1
  • Kit/Kits
  • 3241 INR
    Product Specifications
    • Other
    • AutoPure Magnesium is a reagent kit for direct quantitative determination of magnesium in human serum and plasma on automated clinical chemistry analyzers. AutoPure Magnesium is ready to use, single liquid reagent system.
    • 18 Months
    • Biochemistry Reagent
    • other
    • Other
    • Liquid
    • Biochemistry Reagent
    • Reagents
    • Other
    • ACC9072
    • Industrial
    • Industrial Lab Chemicals
    • 95%
      Product Description
      Introduction
      • AutoPure Magnesium is a reagent kit for direct quantitative determination of magnesium in human serum and plasma on automated clinical chemistry analyzers.
      • AutoPure Magnesium is ready to use, single liquid reagent system.
      • With AutoPure Magnesium, the assay is linear upto 5 mg/dl (2.06 mmo1/1).
      Principle

      • Magnesium ions reacts with xylidyl blue in an alkaline medium to form purple coloured complex.
      • Mg-+ Xylidyl blue -  Magnesium-xylidyl blue complex 
      • The intensity of the purple colour formed is directly proportional to the concentration of magnesium in the specimen and is measured photometrically.
      • Note: Calcium is excluded from the reaction by complexing with EGTA.

      Reagent Storage, Stability & Handling

      AutoPure Magnesium is ready-to-use, single liquid reagent system.

      Shelf life

      Stable till the expiry date indicated on the label, when stored at 2-8C.

      On-Board Reagent Stability

      • 21 days at 2-8 C after opening . Protect the reagent from light and contamination.
      • Do not freeze the reagent.

      Components & Concentration of Working Solution

       Components                                                                     Concentration 

      • Tris buffer; pH 11.0                                                  200 mmol/l
      • EGTA                                                                           60 umol/l
      • Xylidyl blue                                                                     110 mmol/l
      • Stabilizers, excipients & surface active agents

      Specimen Collection & Preservation

      • Collect sample using standard sampling tube. Although serum is  preferred , heparinized plasma can be used. Do not use EDTA plasma.
      • Haemolyzed samples should not be used. Magnesium in serum /plasma is stable for 7 days at 2- 8C and 1year at -20C. Centrifuge samples containing precipitate before performing assay. 

      Calculations

      Fully automated system automatically calculates the Magnesium concentration of each sample.

      • Results in mg/dl = Results in mmo1/1 x 2.43
      • Results in mmo1/1 = Results in mg/di x 0.412
      • Results in mmo1/1 = Results in mEq/I x 0.5
      • Results in mg/di = Results in mEq/I x 1.22

      Application Sheet

      Refer to application sheet for details. For additional system applications, contact our local Accurex representative.

      Calibration

      For calibration, it is recommended to use C.f.a.s* or any other suitable calibrator material.

      Calibration frequency

      Re  calibration is recommended

      • Whenever the reagent lot is changed.
      • As per the requirement of quality control procedures.  

      Quality Control

      Each batch ofAutoPure Magnesium is assayed with atleast six quality control sera  Precinorm, Precipath, Biorad 1 Biorad II Accutrol Normal and Accutrol Abnormal prior to release.

      To ensure adequate quality control, it is recommended that the laboratory should use a normal and abnormal commercial reference control serum. It should be realized that the use of quality control material checks both the reagent and instrument functions together.

      If the control values fall outside the specified limits, each of the below criteria should be cross - checked and corrected:

      • Proper instrument function wavelength setting, light source and temperature control.
      • Cleanliness of probes and cuvettes.
      • Bacterial contamination of wash water used by the instrument.
      • Expiry date of the reagent kit.
      Expected Values

      Serum/ Plasma

      Men

      1.8 -2.6 mg/dl ( 0.74-1.-0 mmol/l)

      Women

      1.9 - 2.5 mg/dl ( 0.78-1.03 mmol/l)

      Children

      1.5 - 2.3 mg/dl ( 0.62-0.95 mmol/l)

      Neonates

      1.2 -2.6 mg/dl ( 0.49 - 1.07 mmol/l)

      Note:

      Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own normal range. For diagnostic purposes, the magnesium results should always be assessed in conjunction with the patient's medical history, clinical examinations and other findings.

      Performance Characteristics Linearity

      With AutoPure Magnesium, the assay is linear upto 5 mg/dl (2.06 mmo1/1). Determine samples with higher concentrations via the rerun function. On instruments without rerun function, manually dilute samples with higher concentrations using 0.9% NaCI or distilled/deionized water (e.g. 1 + 4). Multiply the result by the appropriate dilution factor (e.g. 5).

      Interference

      There is no significant interference in samples containing upto 60 mg/dl of bilirubin. Haemolysis interference is observed since magnesium is released from erythrocytes.

      Precision

       Reproducibility was determined using quality control sera as shown below:

      Within run

      Between run

      Quality Control Material

      Means mg/dl

      SD mg/dl

      CV

      Means mg/dl

      SD mg/dl

      CV

      Low Control Serum

      2.60

      0.061

      2.35

      2.28

      0.051

      2.35

      High Control Serum

      4.16

      0.102

      2.45

      4.30

      0.049

      1.14

       Co - Relation Studies

      A comparison of the magnesium determination using AutoPure Magnesium and reference reagent gave the following co-relation (mg/dl):

      Linear Regression

      • y = 0.945x + 0.2326
      • r= 0.9907
      • Sy.x= 0.16

      No. of samples measured : 62

      The sample concentrations measured were between 1.5 and 5.2 mg/dl.

      References

      •  Tietz, N.W., ed. Clinical Guide to Laboratory Tests, 3rd ed. Philadelphia, Pa : W.B. Saunders, 1995:380-382.
      • Endres DB, Rude RK, Mineral and Bone Metabolism In : Burtis CA, Ashwood ER, editors. Tietz Textbook of Clinical Chemistry, 3rd ed. Philadelphia, WB Saunders Company 1999, p. 1395 -1457.
      • Mann CK,Yoe JH. Spectrophotometric Determination of Magnesium with 1-Azo-2-hydroxy-3-(2,4-dimethyl-carboxanilido)-naphthalene-1'-(2-hydroxy benzene). Anal.Chem. Acta. 1957; 16: 155-60.
      Trade Information
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      • 10000 Per Day
      • 2 Days
      • Yes
      • packaging : Empity
      • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
      • All India
      • certification : iso
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      Phone :+918045479256