Home
Company Profile
Our Products
Contact Us
Select Language 
English
Spanish
French
German
Italian
Chinese (Simplified)
Japanese
Korean
Arabic
Portuguese
Magnesium 80
- Kit/Kits
- 1
- Kit/Kits
- 3241 INR
- Liquid
- Other
- Other
- AutoPure Magnesium is a reagent kit for direct quantitative determination of magnesium in human serum and plasma on automated clinical chemistry analyzers. AutoPure Magnesium is ready to use, single liquid reagent system.
- Biochemistry Reagent
- Biochemistry Reagent
- 18
- other
- Reagents
- Other
- ACC9072
- Industrial
- Industrial Lab Chemicals
- 95%
- AutoPure Magnesium is a reagent kit for direct quantitative determination of magnesium in human serum and plasma on automated clinical chemistry analyzers.
- AutoPure Magnesium is ready to use, single liquid reagent system.
- With AutoPure Magnesium, the assay is linear upto 5 mg/dl (2.06 mmo1/1).
Magnesium ions reacts with xylidyl blue in an alkaline medium to form purple coloured complex.
Mg-+ Xylidyl blue - Magnesium-xylidyl blue complex
The intensity of the purple colour formed is directly proportional to the concentration of magnesium in the specimen and is measured photometrically.
Note: Calcium is excluded from the reaction by complexing with EGTA.
Reagent Storage, Stability & Handling
AutoPure Magnesium is ready-to-use, single liquid reagent system.
Shelf life
Stable till the expiry date indicated on the label, when stored at 2-8C.
On-Board Reagent Stability
21 days at 2-8 C after opening . Protect the reagent from light and contamination.
Do not freeze the reagent.
Components & Concentration of Working Solution
Components Concentration
- Tris buffer; pH 11.0 200 mmol/l
- EGTA 60 umol/l
- Xylidyl blue 110 mmol/l
- Stabilizers, excipients & surface active agents
Specimen Collection & Preservation
Collect sample using standard sampling tube. Although serum is preferred , heparinized plasma can be used. Do not use EDTA plasma.
Haemolyzed samples should not be used. Magnesium in serum /plasma is stable for 7 days at 2- 8C and 1year at -20C. Centrifuge samples containing precipitate before performing assay.
Calculations
Fully automated system automatically calculates the Magnesium concentration of each sample.
Results in mg/dl = Results in mmo1/1 x 2.43
Results in mmo1/1 = Results in mg/di x 0.412
Results in mmo1/1 = Results in mEq/I x 0.5
Results in mg/di = Results in mEq/I x 1.22
Application Sheet
Refer to application sheet for details. For additional system applications, contact our local Accurex representative.
Calibration
For calibration, it is recommended to use C.f.a.s* or any other suitable calibrator material.
Calibration frequency
Re calibration is recommended
- Whenever the reagent lot is changed.
- As per the requirement of quality control procedures.
If the control values fall outside the specified limits, each of the below criteria should be cross - checked and corrected:
- Proper instrument function wavelength setting, light source and temperature control.
- Cleanliness of probes and cuvettes.
- Bacterial contamination of wash water used by the instrument.
- Expiry date of the reagent kit.
Serum/ Plasma
| Men | 1.8 -2.6 mg/dl ( 0.74-1.-0 mmol/l) |
| Women | 1.9 - 2.5 mg/dl ( 0.78-1.03 mmol/l) |
| Children | 1.5 - 2.3 mg/dl ( 0.62-0.95 mmol/l) |
| Neonates | 1.2 -2.6 mg/dl ( 0.49 - 1.07 mmol/l) |
Note:
Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own normal range. For diagnostic purposes, the magnesium results should always be assessed in conjunction with the patient's medical history, clinical examinations and other findings.
Performance Characteristics Linearity
With AutoPure Magnesium, the assay is linear upto 5 mg/dl (2.06 mmo1/1). Determine samples with higher concentrations via the rerun function. On instruments without rerun function, manually dilute samples with higher concentrations using 0.9% NaCI or distilled/deionized water (e.g. 1 + 4). Multiply the result by the appropriate dilution factor (e.g. 5).
Interference
There is no significant interference in samples containing upto 60 mg/dl of bilirubin. Haemolysis interference is observed since magnesium is released from erythrocytes.
Precision
Reproducibility was determined using quality control sera as shown below:
Within run | Between run | |||||
Quality Control Material | Means mg/dl | SD mg/dl | CV | Means mg/dl | SD mg/dl | CV |
Low Control Serum | 2.60 | 0.061 | 2.35 | 2.28 | 0.051 | 2.35 |
High Control Serum | 4.16 | 0.102 | 2.45 | 4.30 | 0.049 | 1.14 |
Co - Relation Studies
A comparison of the magnesium determination using AutoPure Magnesium and reference reagent gave the following co-relation (mg/dl):
Linear Regression
y = 0.945x + 0.2326
r= 0.9907
Sy.x= 0.16
No. of samples measured : 62
The sample concentrations measured were between 1.5 and 5.2 mg/dl.
References
- Tietz, N.W., ed. Clinical Guide to Laboratory Tests, 3rd ed. Philadelphia, Pa : W.B. Saunders, 1995:380-382.
- Endres DB, Rude RK, Mineral and Bone Metabolism In : Burtis CA, Ashwood ER, editors. Tietz Textbook of Clinical Chemistry, 3rd ed. Philadelphia, WB Saunders Company 1999, p. 1395 -1457.
- Mann CK,Yoe JH. Spectrophotometric Determination of Magnesium with 1-Azo-2-hydroxy-3-(2,4-dimethyl-carboxanilido)-naphthalene-1'-(2-hydroxy benzene). Anal.Chem. Acta. 1957; 16: 155-60.
- EXW ABPL
- Paypal, Letter of Credit at Sight (Sight L/C), Letter of Credit (L/C), Cheque, Cash in Advance (CID), Cash Against Delivery (CAD)
- 10000 Per Day
- 2 Days
- Yes
- packaging : Empity
- Africa, Middle East, Western Europe, Eastern Europe, South America, North America, Central America, Australia, Asia
- All India
- certification : iso
212, Udyog Mandir No. 17/C, Bhagoji Keer Marg, Mahim, Mumbai, Maharashtra, 400016, India
Phone :+918045479256