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Phosphorus 100

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Phosphorus 100
Phosphorus 100
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 1015 INR
    Product Specifications
    • other
    • Biochemistry reagent
    • 18
    • Liquid
    • Other
    • Other
    • Auto Zyme Phosphorus is linear up to 20 mg%. Auto Zyme Phosphorus estimates phosphorus in just five minutes. Auto Zyme Phosphorus can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request.
    • Biochemistry reagent
    • Reagents
    • Other
    • ACC74457
    • Industrial
    • Industrial Lab Chemicals
    • 99%
      Product Description
      INTRODUCTION
      • Auto Zyme Phosphorus is a reagent set for determination of Inorganic phosphorus in serum based on UV - End Point method using Ammonium molybdate.
      • Auto Zyme Phosphorus is a single reagent system, ready-to-use.
      • Auto Zyme Phosphorus is linear up to 20 mg%.
      • Auto Zyme Phosphorus estimates phosphorus in just five minutes.
      • Auto Zyme Phosphorus can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request.
      • The shelf-life of Auto Zyme Phosphorus is 18 months.
      PRINCIPLE

      Inorganic Phosphorus reacts with Ammonium molybdate in strong acidic medium to form Phospho-lnorganic Molybdate complex. The absorbance of this complex is directly proportional to the phosphorus concentration.


      PREPARATION OF  WORKING SOLUTION

      The reagent is ready-to-use.

      REAGENT STORAGE & STABILITY

       

      • The reagents are for in vitro diagnostic use.
      • Molybdate reagent & standard should be stored at temperature indicated on the bottle label.

       

      COMPONENTS & CONCENTRATION  OF WORKING SOLUTION

      Component

      Concentration

      Ammonium molybdate

      0.3 mmol/l

      Sulphuric Acid

      1.0%

       SPECIMEN COLLECTION  & PRESERVATION

       

      • Blood should be collected in a clean dry container. Neatly separated serum should be used. Plasma is not recommended as anticoagulants may cause false low results.
      • Phosphorus is stable for 7 days in neatly separated serum. If the estimation is not possible within 7 days then the specimen should be preserved at -10 degree C and should be used within 3 weeks.

       

      PROCEDURE
      • Reaction type: UV - End Point
      • Reaction time:  5 mins. at 37 degree C
      • Wavelength : 340 nm.
      • Zero setting with: Reagent Blank
      • Blank absorbance limit:  0.300 Abs.
      • Sample volume: 0.01 ml (10 ul)
      • Reagent volume: 1.0 ml
      • Standard concentration:  5 mg%
      • Linearity: 20 mg/dl
      Manual assay procedure

      The reagents should be brought to room temperature prior to use. Perform the assay as given below:

      1.0 ml procedure

       

      Serum / Plasma

      Standard

      Blank

      Molybdate Reagent

      0.01 ml

      0.01 ml

       

      1.0 ml

      1.0 ml

      1.0 ml

       

      Incubation

      Incubate the assay mixture for 5 minutes at 37 degree C. After completion of incubation period measure the absorbance specimen and standard against blank at 340 nm. Final colour is stable for two hours if not exposed to direct light.

       

       EXPECTED VALUES

       

      • ADULTS :2.5 - 5.0 mg/dl
      • CHILDREN :4.0 - 7.0 mg/dl

       

      PROCEDURE LIMITATIONS

      • Discard the working reagent if the absorbance of the same is more than 0.300 against distilled water at 340 nm.
      • If the phosphorus value exceeds linearity limit then dilute the specimen suitably with normal saline and repeat the assay. In such case the assay value should be multiplied by the dilution factor to obtain correct phosphorus value of the specimen.
      • Strong lipemic and haemolytic sera should not be used.
      • Contaminated glassware is the greatest source of error. Disposable plastic tubes and clean glasswares are recommended for the test.
      • The reagent contains sulphuric acid. Avoid contact with skin and mucous membrane. If you come in contact with the reagent wash thoroughly with water.
      QUALITY CONTROL

      To ensure adequate quality control, it is recommended that each assay should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might effect the performance of this test include proper instrument function, cleanliness of glassware and accuracy of pipetting.

      Trade Information
      • EXW ABPL
      • Paypal, Letter of Credit at Sight (Sight L/C), Letter of Credit (L/C), Cheque, Cash in Advance (CID), Cash Against Delivery (CAD)
      • 10000 Per Day
      • 2 Days
      • Yes
      • packaging : Empity
      • Africa, Middle East, Western Europe, Eastern Europe, South America, North America, Central America, Australia, Asia
      • All India
      • certification : iso
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      212, Udyog Mandir No. 17/C, Bhagoji Keer Marg, Mahim, Mumbai, Maharashtra, 400016, India
      Phone :+918045479256