0
home

Total Protein 100

home
Total Protein 100
Total Protein 100
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 536 INR
    Product Specifications
    • Biochemistry reagent
    • Other
    • other
    • In - vitro Diagnostics
    • 12 Months
    • Liquid
    • Liquid
    • Reagents
    • Other
    • ACC123
    • Industrial
    • Industrial Lab Chemicals
    • 99%
    Product Description

    INTRODUCTION

    • Auto Zyme Total protein is a reagent set for determination of total protein based on Biuret method.
    • Auto Zyme Total protein is a single reagent system, ready-to-use.
    • Auto Zyme Total protein is linear up to 18 gm%.
    • Total protein can be determined in just 5 minutes.
    • Auto Zyme Total protein does not require serum blank for lipemic samples.
    • Auto Zyme Total protein can be used on any Colorimeter, Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request.
    • The shelf-life of Auto Zyme Total protein is 18 months.

    PRINCIPLE

    Proteins react with cupric ions under alkaline pH to produce a colour complex. This colour complex absorbs light at 546 nm. (530-570 nm.). The intensity of the colour is directly proportional to the protein concentration in specimen.


    PREPARATION OF  WORKING SOLUTION

    The reagent is ready-to-use.

    REAGENT STORAGE & STABILITY

     

    • The reagents are for in vitro diagnostic use.
    • Biuret reagent & standard should be stored at temperature indicated on the bottle label.

    COMPONENTS & CONCENTRATION  OF WORKING SOLUTION

     

    Component

    Concentration

    Cupric sulphate

    7 mmo1/1

    Potassium Iodide

    6 mmo1/1

    Tartarate

    20 mmo1/1

    Surfactant

    0.05% w/v

    Stabilizer

    7 mmo1/1

    SPECIMEN COLLECTION  & PRESERVATION

    Blood should be collected in a clean dry container. Avoid the use of plastic or siliconized containers for blood collection which may prolong clotting time. Serum or plasma should be separated from the cells within 60 minutes.

    PROCEDURE

     

    • Reaction type: End-Point
    • Reaction time: 5 mins. at 37 degree C
    • Wavelength:546 nm.(530-570 nm.)
    • Zero setting with : Reagent Blank
    • Sample volume:  0.01 ml (10 III)
    • Reagent volume: 1.0 ml
    • Standard concentration:  6 gm%
    • Linearity: 18 gm%

     

    Manual assay procedure

    Prewarm at room temperature (25-30 degree C) the required amount of working solution before use.


    Perform the assay as given below :

    1.0 ml procedure

     

    Serum / Plasma

    Standard

    Blank

    Working Solution

    0.01 ml

    0.01 ml

     

    1.0 ml

    1.0 ml

    1.0 ml

     Incubation

    Incubate the assay mixture for 5 minutes at 37 degree C. After completion  of incubation period measure the absorbance of specimen and  standard against blank. Final colour is stable for 8 hours if not exposed to direct light.

    PROCEDURE LIMITATIONS

    • The working reagent is considered unsatisfactory and should not be used if it develops insoluble precipitate.
    • If the total protein content of a specimen exceeds 18 gm% dilute the specimen with an equal volume of saline and repeat the assay. Multiply the result by two to obtain correct value.
    • For Haemolyzed and Icteric samples (Bilirubin > 5 mg%) a saline blank should be run along with the assay. Read the absorbance of saline blank against distilled water and subtract it from sample absorbance.

    QUALITY CONTROL

    To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realised that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.


    Trade Information
    • EXW ABPL
    • Letter of Credit at Sight (Sight L/C), Letter of Credit (L/C), Cheque, Cash in Advance (CID), Cash Against Delivery (CAD)
    • 10000 Per Day
    • 2 Days
    • Yes
    • packaging : Empity
    • All India
    • certification : iso
      Related Products
      HDL Cholesterol Direct 80
















      Send Inquiry
      Autopure - T-LDL Cholestrol-Calibrator
















      Send Inquiry
      Autopure  T HDL Cholestrol Calibrator















      Send Inquiry
      Lipase 25













      Send Inquiry
      HDL Cholesterol Direct 80













      Send Inquiry
      Bilirunin (T And D) 200





      Send Inquiry
      right arrow
      left arrow
      Contact Us

      212, Udyog Mandir No. 17/C, Bhagoji Keer Marg, Mahim, Mumbai, Maharashtra, 400016, India
      Phone :+918045479256