Total Protein 80

Total Protein 80
Total Protein 80
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 670 INR
    Product Specifications
    • 18 Months
    • Biochemistry Reagent
    • other
    • Liquid
    • Other
    • Other
    • AutoPure Total Protein is ready to use, single liquid reagent system. With AutoPure Total Protein the assay is linear upto 18 g/dl (180 g/l)
    • Biochemistry Reagent
    • Reagents
    • Other
    • ACC90677
    • Industrial
    • Industrial Lab Chemicals
    • 95%
      Product Description
      • AutoPure Total Protein is a reagent kit for direct quantitative determination of total protein in human serum and plasma on automated clinical chemistry analyzers.
      • AutoPure Total Protein is ready to use, single liquid reagent system.
      • With AutoPure Total Protein the assay is linear upto 18 g/dl (180 g/l).

      Divalent copper ion reacts in alkaline solution with protein peptide bonds to form purple coloured biuret complex.

      Protein + Cu2+ - Cu - protein complex. 

      The intensity of purple colour formed is directly proportional to the concentration of the total protein in the specimen and is measured photometrically.

      Reagent Storage, Stability & Handling

      AutoPure Total Protein is ready to use, single liquid reagent system.

      Shelf life

      Stable till the expiry date indicated on the label, when stored at 15-25 C.

      On  Board Reagent Stability.

      45 days at 2- 8C after opening. Protect the reagent from light and contamination.

      Do not freeze the reagent.

      Components & Concentration of Working Solution 

       Components                                                                         Concentration


      • Cupric sulphate                                                               7 mmol/l                                                      
      • Potassium iodide                                                         6 mmol/l
      • Sodium Potassium tartarate                                        40 mmol/l
      • Sodium hydroxide                                                       700 mmol/l
      • Stabilizers, excipients   surface active agents


      Specimen Collection & Preservation
      Collect sample using standard sampling tube. Serum, heparinized plasma or EDTA plasma can be used. Using plasma, results could be falsely elevated upto 0.4 g/dI due to fibrinogen. Avoid haemolysis since haemoglobin reacts as protein in the total protein assay.

      Total protein in serum / plasma is stable for 3 days at 2- 8C and 6 months at  -20C. Centrifuge samples containing precipitate before performing assay.


      Fully automated system automatically calculates the total protein concentration of each sample.

      Results in g/I = Results in g/dI x 10

      Application Sheet

      Refer to the application sheet for details. For additional system applications, contact our local Accurex representative.


      For calibration, it is recommended to use C.f. a.s or any other suitable calibrator material.

      Calibration frequency

      Re  calibration is recommended


      • Whenever the reagent lot is changed.
      • As per the requirement of quality control procedures 


       Quality Control

      Each batch of AutoPure Total Protein is assayed with atleast six quality control sera   Precinorm, Precipath, Biorad I , Biorad 11, Accutrol Normal and Accutrol Abnormal prior to release

      To ensure adequate quality control, it is recommended that the laboratory should use a normal and abnormal commercial reference control serum. It should be realized that the use of quality control material checks both the reagent and instrument functions together.

      If the control values fall outside the specified limits, each of the below criteria should be cross - checked and corrected:


      • Proper instrument function  wavelength setting, light source and temperature control.
      • Cleanliness of probes and cuvettes.
      • Bacterial contamination of wash water used by the instrument.
      • Expiry date of the reagent kit.


      Expected Values
      Serum/ Plasma
      Adults : 6.3 - 8.4 g/dI (63  - 84 g/1)

      Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own normal range. For diagnostic purposes, the total protein results should always be assessed in conjunction with the patient's medical history, clinical examinations and other findings.

      Performance Characteristics Linearity

      With AutoPure Total Protein, the assay is linear up to 18 g/dI (180 g/l). Determine samples with higher concentrations via the rerun function. On instruments without rerun function, manually dilute samples with higher concentrations using 0.9% NaCI or distilled/deionized water (e.g. 1 + 3). Multiply the result by the appropriate dilution factor (e.g. 4).


      There is no significant interference in samples containing upto 40 mg/di of bilirubin and 500 mg/di of haemoglobin. Haemoglobin interferes in the results as it reacts as a protein in total protein assay.


      Reproducibility was determined using quality control sera as shown below:

      Within run

      Between run

      Quality control material

      Mean g/dl

      SD g/dl


      Mean g/dl


      SD g/d


      low control serum







      High control serum








       Co - Relation Studies

      A comparison of the Total Protein determination using AutoPure Total Protein reagent and AutoZyme Total Protein gave the following co relation (g/dI):

      Linear Regression

      y = -0.201 + 1.030x

      r = 0.996

      Sy.x = 0.10

      No. of samples measured : 85

      The sample concentrations measured were between 4 g/dI and 9 g/dl.



      • Tietz, N.W., ed. Clinical Guide to Laboratory Tests, 3rd ed. Philadelphia, Pa : W.B. Saunders, 1995: 518-522
      • Koller, A. Total Serum Protein. In Kaplan LA, Pesce AJ, eds. Clinical Chemistry Theory, Analysis and Correlation. St. Louis : Mosby Company, 1984 :1316 -1319.
      • Henri, R.J., et al "Clinical Chemistry - Principles and Techniques Harper & Row II ed (1974).
      • Flack, C.P. and Woolen, J.W. Clin Chem. 30,559 (1984).

      Trade Information
      • EXW ABPL
      • Paypal, Letter of Credit at Sight (Sight L/C), Letter of Credit (L/C), Cheque, Cash in Advance (CID), Cash Against Delivery (CAD)
      • 10000 Per Day
      • 2 Days
      • Yes
      • packaging : Empity
      • Africa, Middle East, Western Europe, Eastern Europe, South America, North America, Central America, Australia, Asia
      • All India
      • certification : iso
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      Contact Us

      212, Udyog Mandir No. 17/C, Bhagoji Keer Marg, Mahim, Mumbai, Maharashtra, 400016, India
      Phone :+918045479256