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Triglyceride 50

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Triglyceride 50
Triglyceride 50
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 1823 INR
    Product Specifications
    • Liquid
    • Other
    • Biochemistry Reagent
    • Other
    • other
    • 18 Months
    • Triglycerides can be determined in just 10 minutes at 37 degree C or 20 minutes at R.T. (25 degree C - 30 degree C). Infinite Liquid Triglycerides is linear upto 800 mg%. Infinite Liquid Triglycerides can be used on any Colorimeter, Spectrophotometer, Discrete semiautomated and Automated analyzer. Programme can be designed for any specific analyzer upon request.
    • Biochemistry Reagent
    • Reagents
    • Other
    • AC90800
    • Industrial
    • Industrial Lab Chemicals
    • 99%
      Product Description
      INTRODUCTION
      • Infinite  Liquid Triglycerides is a reagent set for determination of triglycerides, based on enzymatic method using Lipoprotein lipase, Glycerol kinase, Glycerol phosphate oxidase and Peroxidase.
      •  Infinite  Liquid Triglycerides is a ready - to - use reagent.
      • Triglycerides can be determined in just 10 minutes at 37 degree C or 20 minutes at R.T. (25 degree C - 30 degree C).
      •  Infinite  Liquid Triglycerides is linear upto 800 mg%.
      •  Infinite  Liquid Triglycerides can be used on any Colorimeter, Spectrophotometer, Discrete semiautomated and Automated analyzer. Programme can be designed for any specific analyzer upon request.
      • The influence of lipids, haemolysis and bilirubin (upto 8 mg%) is negligible.
      PRINCIPLE

      Glycerol released from hydrolysis of triglycerides by lipoprotein lipase is converted by glycerol kinase into glycerol - 3 - phosphate which is oxidised by glycerol phosphate oxidase to dihydroxyacetone phosphate and hydrogen peroxide. In presence of peroxidase, hydrogen peroxide oxidizes phenolic chromogen to a red coloured compound.

      REAGENT STORAGE, STABILITY & HANDLING

      • The kit should be stored at 2 - 8 degree G and is stable till the expiry date indicated on the label.
      • The reagent and standard are ready-to-use and are stable till expiry, when stored at 2 -8 degree C. DO NOT FREEZE THE REAGENT.
      • The reagent should be stored only in the amber bottle provided to protect it from direct light. Before use swirl in the reagent gently. DO NOT SHAKE VIGOROUSLY.
      • Overtime, the reagent may develop a light pink colour. This is expected and does not affect the reagent performance. Discard the reagent if the absorbance of the same exceeds 0.300 O.D. against distilled water at 510 nm.
      • Contamination of the reagent should be strictly avoided. Should the reagent develop turbidity discard the reagent.

      COMPONENTS & CONCENTRATION OF WORKING SOLUTION

      Component

      Concentration

      Buffer, pH 7.2

      50 mmol/I

      Lipase

      2000 IU/I

      Glycerol kinase

      300 IU/I

      Glycerol phosphate oxidase

      1000 IU/I

      Peroxidase

      500 IU/I

      ATP

      1 mmol/I

      Chromogen

      2 mmol/I

       NOTE :

      • The specimen to reagent ratio can be altered proportionally without affecting the results.
      • Falsely elevated results are obtained due to lipemic samples. The   Liquid Triglycerides overcomes falsely elevated result with use of Lipid clearing agent in the reagent formulation. Lipid clearing agent clears up the turbidity caused by lipemic samples
      SPECIMEN COLLECTION & PRESERVATION

      Blood should be collected in a clean dry container. Avoid the use of plastic or siliconized container which may prolong clotting time. Serum or plasma should be separated from the cells at the earliest possible (within 30 minutes). For plasma collection following anticoagulants may be used.
      • EDTA 2 mg/ml of blood
      • CITRATE 6 mg/ml of blood
      • HEPARIN 200 IU/ml of blood
      Avoid use of Oxalate and Sodium Fluoride as anticoagulant. Triglycerides are stable for 4 days in neatly separated serum or plasma at2-8 degree C.

      PROCEDURE
      • Reaction type: End-Point
      • Reaction time: 10 mins. at 37 degree C/20 mins. at R.T. (25 -30 degree C)
      • Wavelength: 510 nm. (500 - 530 nm.)
      • Zero setting with: Reagent Blank
      • Blank absorbance limit: 0.300 Abs.
      • Sample volume : 0.01 ml (10 (If)
      • Reagent volume: 1.0 ml
      • Standard concentration: 200 mg%
      • Linearity: 800 mg/dl
      Manual assay procedure

      Prewarm at room temperature (25-30 degree C) the required

      Amount of reagent before use. Perform the assay as given below

      1.0 ml procedure

       

      Serum / Plasma

      Standard

      Blank

      Reagent

      0.01 ml

      0.01 ml

       

      1.0 ml

      1.0 ml

      1.0 ml

      Incubation

      Incubate the assay mixture for 10 minutes at 37 degree C or 20 minutes at

      R.T. (25 -30 degree C). After incubation, measure the absorbance against blank at 510 nm. (500-530 nm.). Final colour is stable for 30 minutes if not exposed to direct light.

      EXPECTED VALUES

      Upto 170 mg%

      NOTE :

      Expected range varies from population to population therefore each laboratory should establish its own normal range.

      PROCEDURE LIMITATIONS

      • If the triglycerides value exceeds 800 mg% dilute the specimen with saline(1:1 ratio) and repeat the assay. Multiply the result obtained by 2 to get the correct triglycerides value.
      • Glycerol contamination in glassware leads to erroneous results.
      • Care should be taken not to touch reagent and samples with fingers, especially after applying hand lotion which may contain glycerine.

      QUALITY CONTROL

      To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realised that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting

      Trade Information
      • EXW ABPL
      • 10000 Per Day
      • 2 Days
      • Yes
      • packaging : Empity
      • Australia, North America, South America, Eastern Europe, Western Europe, Middle East, Africa, Asia, Central America
      • All India
      • certification : iso
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