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Urea 200

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Urea 200
Urea 200
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 1926 INR
    Product Specifications
    • Biochemistry reagent
    • Biochemistry reagent
    • Other
    • 18 Months
    • other
    • Other
    • Auto Zyme urea can be used on any Colorimeter, Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request. Auto Zyme Urea has one step reconstitution.
    • Liquid
    • Reagents
    • Other
    • ACC96698
    • Industrial
    • Industrial Lab Chemicals
    • 99%
      Product Description

      INTRODUCTION

      • Auto Zyme Urea is a reagent set for determination of Urea / Blood Urea Nitrogen based on enzymatic method using Urease.
      • Auto Zyme Urea is a two reagent system using two step procedure.
      • Auto Zyme Urea is linear up to 350 mg%.
      • The stability of enzyme solution is 6 months at 2 -8 degree C
      • Auto Zyme urea can be used on any Colorimeter, Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request.
      • Auto Zyme Urea has one step reconstitution.
      • Urea can be determined in just 8 minutes.
      • Accuracy, precision, specificity and sensitivity is better than that of chemical method like : Diacetyl monoxime (DAM) and NED.
      • The shelf-life of Auto Zyme Urea is 18 months.

      PRINCIPLE


      Urease splits urea into ammonia and carbondioxide. Ammonia released in this reaction reacts with hypochlorite and phenolic chromogen to produce green colour. The absorbance of this green colour at 578 nm. (570-620) is directly proportional to the concentration of urea in specimen.

      PREPARATION OF  ENZYME SOLUTIONS

      Reconstitute enzyme & diluent as per instructions indicated on individual bottle label to prepare enzyme solution. Mix gently by inversion. The chromogen solution is ready-to- use.

      REAGENT STORAGE & STABILITY

      The reagent kit should be stored at 2-8 degree C and is stable till the expiry date indicated on the label. The reconstituted enzyme solution is stable for 6 months when stored at 2-8 degree C. DO NOT FREEZE THE ENZYME SOLUTION. The chromogen solution is stable for 6months at 2-8 degree C after opening.

      COMPONENTS & CONCENTRATION  OF WORKING SOLUTION

      Component

      Concentration

      Phosphate buffer; pH 7.0

      35 mmo1/1

      Urease

      15 KU/I

      Phenolic Chromogen

      2 mmo1/1

      Hypochlorite

      4 mmo1/1

      SPECIMEN COLLECTION  & PRESERVATION

      Blood should be collected in a clean and dry container (free of NH3 ). Avoid the use of plastic or siliconized containers which may prolong clotting time.

      Following anticoagulants may be used for plasma separation:

       

      • EDTA: 2 mg/ml of blood
      • CITRATE: 6 mg/ml of blood
      • OXALATE: 3 mg/ml of blood
      • HEPARIN: 200 IU/m1 of blood

       

      Ammonium salts of anticoagulants and sodium fluoride should not be used as anticoagulant.

      Urea in the specimen is stable for a week when stored at 2-8 degree C and for a month when stored at -10 degree C.

      PROCEDURE

      • Reaction type: End-Point
      • Reaction time : 3 + 5 min.
      • Wavelength:578 nm. (570 - 620 nm.)
      • Zero setting with: Reagent Blank
      • Blank absorbance limit : 0.200 Abs.
      • Sample volume: 0  01 ml (100)
      • Reagent volume: 1  0 ml + 1.0 ml
      • Standard concentration: 40 mg%
      • Linearity : 350 mg/dl
      Prewarm at room temperature (25 - 30 degree C) the required amount of Enzyme solution and chromogen solution before use.


      Perform the assay as given below :

      2.0 ml procedure

      Manual assay procedure

       

      Serum / Plasma

      Standard

      Blank

      Enzyme Solution

      0.01 ml

      0.01 ml

       

      1.0 ml

      1.0 ml

      1.0 ml

      Mix and incubate for three minutes at 37 degree C.

      Chromogen Solution

      1.0 ml

      1.0 ml

      1.0 ml

      Incubation

      Mix and incubate the assay mixture at 37 degree C for 5 minutes. After completion of incubation measure the absorbance of assay mixture against blank at 578 nm. (570-620 nm.). The final colour is stable for 2 hours if not exposed to direct light.

      EXPECTED VALUES

      • Urea: 10to45 mg%
      • Urea Nitrogen: 5to21 mg%

      Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own normal range.

      PROCEDURE LIMITATIONS

       

      • Fluoride as an anticoagulant cannot be used as it inhibits urease activity. Anticoagulants having ammonium ions should not be used because of extreme sensitivity of the colour reaction to ammonia.
      • If the urea value exceeds 350 mg% then dilute the specimen suitably with normal saline. In such case, the results obtained should be multiplied with the dilution factor to obtain the correct urea value.
      • During assay, blank develops a prominent yellow colour. Only if the absorbance of the same exceeds 0.200 at 578 nm. against distilled water, the reagents should be considered unsatisfactory and should not be used.
      • Care should be taken so as not to contaminate the reagents by interchanging the pipettes of enzyme solution & chromogen.
      • Detergents containing ammonium ions & strong oxidizing disinfectants (sodium hypochlorite) should not be used for washing glass wares.

       

      QUALITY CONTROL

      To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might effect the performance of this test include proper instrument function,temperature control, cleanliness of glassware and accuracy of pipetting.


       




      Trade Information
      • EXW ABPL
      • Paypal, Cheque, Cash in Advance (CID), Cash Advance (CA), Cash Against Delivery (CAD)
      • 10000 Per Day
      • 2 Days
      • Yes
      • packaging : Empity
      • All India
      • certification : iso
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        212, Udyog Mandir No. 17/C, Bhagoji Keer Marg, Mahim, Mumbai, Maharashtra, 400016, India
        Phone :+918045479256