Uric Acid 200

Uric Acid 200
Uric Acid 200
Price And Quantity
  • Kit/Kits
  • 1
  • Kit/Kits
  • 5402 INR
    Product Specifications
    • 18 Months
    • Other
    • Biochemistry Reagent
    • other
    • Other
    • AutoPure Uric Acid is a ready to use, single liquid reagent system. With AutoPure Uric Acid the assay is linear upto 25 mg/dl (1.48,,ol/l).
    • Liquid
    • Biochemistry Reagent
    • Reagents
    • Other
    • ACC9073
    • Industrial
    • Industrial Lab Chemicals
    • 95%
      Product Description


      • AutoPure Uric Acid is a reagent kit for direct quantitative determination of  Uric Acid in human serum and plasma on automated clinical chemistry analyzers.
      • AutoPure  Uric Acid is a ready to use, single liquid reagent system.
      • With   AutoPure Uric Acid  the assay is linear upto 25 mg/dl (1.48,,ol/l).


      Uricase cleaves uric acid form allantoin and hydrogen peroxide.

      Uric acid + H2O+ O2 Allantoin + H2O2+ CO2

      In presence of peroxidase , hydrogen peroxide oxidatively couples with 4 aminoantipyrine and DHBS to produce red quinoneimine dye. The intensity of the red colour formed is directly proportional to the concentration of the uric acid in the specimen and is measured photometrically.

      H2O2+4- aminoantipyrine + DHBS -Red quinoneimine dye +H20

      DHBS = 3,5 - dichloro-2- hydroxy benzene sulfonic acid 

      Reagent  Storage Stability & Handling 

       AutoPure Uric acid is a ready to use , single liquid reagent system. 

      Shelf life 

      Stable till the expiry date  indicated on the label,when stored at 2 C - 8 C. 

      On- Board Reagent Stability

       45days at 2- 8 C  after opening. Protect he reagent from light and contamination.

      Over a period of time, the reagent may develop a light pink colour. This is expected and does not affect the reagent performance. Discard the reagent if the absorbance of the same exceeds 0.300 O.D. against distilled water at 505nm(500-530nm).

      Do not freezethe reagent 

      Components & Concentration of Working Solution 

       Components                                                                                     Concentration 


      •  HEPES buffer, pH 7.8                                                              50 mmol/l
      • Peroxidase                                                                                >2500IU/I
      • Uricase                                                                                       >400IU/I
      • Ascorbate oxidase                                                                       >300IU/I
      • 4- aminoantipyrine                                                                        > 0.1 mmol/l
      • DHBS                                                                                             >1mmol/l
      • Stabilizers, excipients & surface active agents 

      Specimen Collection&  Preservation

      Collect sample using standard sampling tube. Serum or heparinized plasma or EDTA plasma can be used . Uric acid in serum/ plasma is stable for 5 days at 2- 8 C and 6 month at -20 C . Centrifuge samples containing precipitate before performing assay.



       Fully automated system automatlcally calculated the Uric acid concentration of each sample.

      Results in mmol/l= Result in mg/dlx 0.059

       Results in mmol/l= Result in mmolx 16.81

      Results in umol/l= Result in mg/dlx 59.5

      Application Sheet 

      Refer to the application sheet for details. For additional system application , contact our local Accurex representative.


       For calibration , it is recommended to use C.f a,s or any other suitable calibrator material.

      Calibration frequency  

      Re- calibration is recommended 

      • Whenever the reagent lot is changed  .
      • As per the requirement of quality control procedures.

      Quality Control

      Each batch of AutoPure Uric Acid is assayed with atleast six quality control sera- percinorm, Precipath Biorad l bioradll, Accutrol Normal and Accutrol Abnormal prior to release.

      To ensure adequate quality control , it is recommended that  the laboratory should use a normal and abnormal commerial reference control serum. It should be realized that  the use of quality control material checks both the reagent and instrument functions together.

      If the control values fall outside the specified limits, each of the below criteria should be cross- checked and corrected: 

      • Proper instrument function- wavelength setting , light source and temperature control.
      • Cleanliness of probes and cuvettes .
      • Bacterial contamination of wash water used by the instrument.
      • Expiry date of the reagent kit. 

      Expected Values 



      3.4- 7.0 mg/dl/ 202.3- 416.5 umol/l / 0.20 - 0.41 mmol/l


      2.4- 5.7 mg/dl/ 142. 8 - 339.2 umol/l 0.14 - 0.34, mmol/l


      Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own range. For diagnostic purposes, the a-Amylase results should always be assessed in conjunction with the patients medical history, clinical  examination and other findings.

      Performance Characteristic


      With AutoPure Uric Acid , the assay is linear upto 25 mg/dl( 1.48 mmol/l). Determine samples with higher concentrations via the rerun function .On instruments without rerun function , manually dilute samples with higher concentrations using 0.9 % Nacl or distilled/ deionized water (e.g. 1 +1 ) Multiply the result by the appropriate dilution factor(eg. 2).


      There is no signifcant interference   in samples containing upto 40 mg/dl of  billrubin and 500 mg/dl of haemoglobin. Ascorbic acid <30 mg/dl does not  interfere. Of the drugs tested in vitro, a- methydopa, desferoxamine and calcium dobesilate (eg Dexium) cause interference at therapeutic concentrations (uric acid level artificially low). Uricase reacts speciically with the uric acid. Other purine derivatives can in hibit the uric acid reaction.


       Reproducibility was determined using quality control sera as shown  below:

      Within run

      Between run


      Quality control material

      Mean lu/l

      SD IU/I


      Mean lu/l

      SD IU/I


      Low control serum







      High control serum







       Co- Relation Studies

      A comparison of the Uric Acid  determination using AutoPure Uric Acid  and Infinite a Uric Acid  reagent gave the following co relation (mg/dl):

      Trade Information
      • EXW ABPL
      • Letter of Credit at Sight (Sight L/C), Letter of Credit (L/C), Cheque, Cash in Advance (CID), Cash Against Delivery (CAD)
      • 10000 Per Day
      • 2 Days
      • Yes
      • packaging : Empity
      • Africa, Middle East, Western Europe, Eastern Europe, South America, North America, Central America, Australia, Asia
      • All India
      • certification : iso
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